多指标正交试验优选莲松异搏停片中延胡索、苦参醇提工艺

李艳英; 王超; 郭小玉; 张永丽; 黄能听

doi:10.13422/j.cnki.syfjx.2014130026

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多指标正交试验优选莲松异搏停片中延胡索、苦参醇提工艺

工艺与制剂 | 更新时间：2024-01-04

- 多指标正交试验优选莲松异搏停片中延胡索、苦参醇提工艺
- Optimization of Extraction Technology for Corydalis Rhizoma and Sophorae Flavescentis Radix in Liansong Yiboting Tablets by Orthogonal Design with Multi-indexes
- 中国实验方剂学杂志 2014年20卷第13期页码：26-28
- 作者机构：
- 作者简介：
- 基金信息：
  
  北京市科委“十病十药”研发项目（Z121102001112009）
- DOI：10.13422/j.cnki.syfjx.2014130026
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
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李艳英, 王超, 郭小玉, 等. 多指标正交试验优选莲松异搏停片中延胡索、苦参醇提工艺[J]. 中国实验方剂学杂志, 2014,20(13):26-28.

LI Yan-ying, WANG Chao, GUO Xiao-yu, et al. Optimization of Extraction Technology for Corydalis Rhizoma and Sophorae Flavescentis Radix in Liansong Yiboting Tablets by Orthogonal Design with Multi-indexes[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 26-28.
李艳英, 王超, 郭小玉, 等. 多指标正交试验优选莲松异搏停片中延胡索、苦参醇提工艺[J]. 中国实验方剂学杂志, 2014,20(13):26-28. DOI： 10.13422/j.cnki.syfjx.2014130026.

LI Yan-ying, WANG Chao, GUO Xiao-yu, et al. Optimization of Extraction Technology for Corydalis Rhizoma and Sophorae Flavescentis Radix in Liansong Yiboting Tablets by Orthogonal Design with Multi-indexes[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 26-28. DOI： 10.13422/j.cnki.syfjx.2014130026.

摘要

目的：优选莲松异搏停片中延胡索、苦参的提取工艺。方法：以延胡索乙素、苦参碱和氧化苦参碱提取量为指标，通过正交试验和单因素试验考察乙醇体积分数、溶剂用量、提取次数和时间对醇提工艺的影响。采用HPLC测定指标成分含量，流动相乙腈（A）-磷酸二氢铵溶液（B）梯度洗脱（0~10 min，10%A；10~25 min，10%~37%A；25~45 min，37%A），检测波长220 nm。结果：最佳提取工艺为加6倍量70%乙醇提取3次，每次2 h；延胡索乙素提取量332.04 mg，苦参碱和氧化苦参碱总量3 192.08 mg。结论：优选的提取工艺稳定可行，建立的色谱条件可较好地分离3个指标成分。

Abstract

Objective: To optimize extraction technology of Corydalis Rhizoma and Sophorae Flavescentis Radix in Liansong Yiboting tablets. Method: With extracting amounts of matrine

oxymatrine and tetrahydropalmatine as indexes

orthogonal test and single factor test was adopted to optimize extraction process by taking ethanol concentration

solid-liquid ratio

extracting times and time as factor.HPLC was employed to determine contents of index components with mobile phase of acetonitrile(A)-ammonium dihydrogen phosphate solution(B) for gradient elution(0-10 min

10%A;10-25 min

10%-37%A;25-45 min

37%A) and detection wavelength at 220 nm. Result: Optimum extraction technology was as following:extracted 3 times with 6 times the amount of 70% ethanol for 2 h each time.Extracting amount of tetrahydropalmatine was 332.04 mg

total extracting amount of matrine and oxymatrine was 3 192.08 mg. Conclusion: This optimized extraction technology was stable and feasible

these established chromatographic conditions had a good separation for three components.

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