大孔树脂分离纯化白花前胡乙素的工艺优选

聂小忠; 党建章; 王晓利

doi:10.13422/j.cnki.syfjx.2014130035

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大孔树脂分离纯化白花前胡乙素的工艺优选

工艺与制剂 | 更新时间：2024-01-04

- 大孔树脂分离纯化白花前胡乙素的工艺优选
- Optimization of Separation and Purification Process for Praeruptorin B from Peucedani Radix with Macroporous Resin
- 中国实验方剂学杂志 2014年20卷第13期页码：35-37
- 作者机构：
- 作者简介：
- 基金信息：
  
  深圳职业技术学院校级科研基金重点项目（601422K27001）
- DOI：10.13422/j.cnki.syfjx.2014130035
  中图分类号： O657.72;R284.2
- 纸质出版日期：2014
- 稿件说明：
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聂小忠, 党建章, 王晓利. 大孔树脂分离纯化白花前胡乙素的工艺优选[J]. 中国实验方剂学杂志, 2014,20(13):35-37.

NIE Xiao-zhong, DANG Jian-zhang, WANG Xiao-li. Optimization of Separation and Purification Process for Praeruptorin B from Peucedani Radix with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 35-37.
聂小忠, 党建章, 王晓利. 大孔树脂分离纯化白花前胡乙素的工艺优选[J]. 中国实验方剂学杂志, 2014,20(13):35-37. DOI： 10.13422/j.cnki.syfjx.2014130035.

NIE Xiao-zhong, DANG Jian-zhang, WANG Xiao-li. Optimization of Separation and Purification Process for Praeruptorin B from Peucedani Radix with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 35-37. DOI： 10.13422/j.cnki.syfjx.2014130035.

摘要

目的：优选信前胡的大孔树脂纯化工艺条件。方法：以白花前胡乙素提取量为评价指标，通过正交试验考察乙醇体积分数、溶剂用量、提取时间对信前胡提取工艺的影响。通过单因素试验筛选大孔树脂型号并考察大孔树脂吸附与洗脱条件。采用HPLC测定白花前胡乙素含量，流动相甲醇-水（75：25），检测波长321 nm。结果：最佳提取工艺为加8倍量60%乙醇提取3.5 h。选择X-5型大孔树脂，吸附时间60 min，白花前胡乙素吸附率92.36%，洗脱率71.24%，提取率98.71%，纯度8.88%。结论： X-5型大孔树脂适合分离纯化白花前胡乙素，为信前胡香豆素类成分的开发应用提供参考。

Abstract

Objective: To optimize purification process of praeruptorin B from Peucedani Radix by macroporous resin. Method: With extracting amount of praeruptorin B as index

orthogonal test was adopted to investigate effects of ethanol concentration

the amount of solvent and extracting time on extraction process.Type of macroporous resin and its elution conditions were investigated by single factor tests.HPLC was employed to determine the content of praeruptorin B with mobile phase of methanol-water(75:25) and detection wavelength at 321 nm. Result: Optimum extraction technology was as following:extracted 3.5 h with 8 times the amount of 60% ethanol.X-5 macroporous resin was selected

its adsorption time was 60 min

adsorption rate

elution rate

recovery and purity were 92.36%

71.24%

98.71% and 8.88%

respectively. Conclusion: X-5 macroporous resin was suitable for separation and purification of praeruptorin B

this study could provide a reference for development and applicaiton of total coumarins from Peucedani Radix.

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