Objective: To establish a sensitive HPLC fingerprint analytical method for evaluating scientifically and controlling the quality of Xiaoerqixingcha granule manufactured by Guangzhou wanglaoji Pharmaceutical Company Limited. Method: RP-HPLC analysis was performed at 30 ℃ by using a Symmetry® C18 column(4.6 mm×250 mm

5 μm)

with the gradient elute of mobile phase consisting of acetonitrile-0.2% glacial acetic acid at the flow rate of 1.0 mL·min-1.The UV detection was set at 254 nm. Result: Liquiritin was used as the reference substance in the HPLC chromatographic fingerprint of Xiaoerqixingcha Granule

which showed 20 common peaks and the similarity was more than 0.90 among 10 samples. Conclusion: This method was accurate

repeatable and useful for the quality control of Xiaoerqixingcha granules of Guangzhou wanglaoji Pharmaceutical Company Limited.