Objective: The methodology study was conducted in RBL-2H3 cells allergy test

and its applicability into evaluating anaphylactoid reactions was studied. Method: The grown characteristics of RBL-2H3 cells were observved

then compound 48/80(C48/80) was used to optimize the experimental conditions such as cell density

concentration of dosage

testing indexes.The application of the method was studied though testing two reported allergic reaction drugs

which was used to evaluate two herbs injections. Result: As a vitro test to detect drug allergic reaction

the optimum planting density of RBL-2H3 test was (0.5-2.0) ×105/mL

optimal incubation time was 24 h

optimal drug interaction medium was Tyrode's solution; the optimal condition of C48/80 induced RBL-2H3 cell degranulation test was 25-50 mg·L-1 concentration and response 30 min;C48/80

Tween-80 and Chinese cobra toxin could induce RBL-2H3 cell degranulation and the increase of cell supernatant β-glucosidase and histamine concentrations

which showed a good‘quantity-time-effect’relationship. For Xuesaitong the potential anaphylactoid risk was found. Conclusion: As a in vitro test for drug anaphylactoid

RBL-2H3 cell method is sensitive

rapid and convenient

it suits the anaphylactoid testing of drugs including TCM injections.For non-ferrous subjects content

it is suggested that the index of histamine ELISA method

confirm at last still need to be combined with experiment in vivo.