Objective: To explore structure of polypeptides from Cervi Cornu Pantotrichum-collagen/chitosan composite materials prepared by different crosslinking methods and investigate its effects on osteoblasts proliferation. Method: Mixed collagen and chitosan solution at the ratio of 9:1

vacuum thermo-crosslinking and 0.25% glutaraldehyde cross-linking method were used to prepare composite scaffolds which contained with 1.0 mg·L-1 of polypeptides from Cervi Cornu Pantotrichum.Structure of composite scaffolds was observed by scanning electron microscopy

MTT assay were used to test effects of different composite materials on osteoblast hFOB1.19 proliferation and activity of alkaline phosphatase(ALP). Result: Structure of thermo-crosslinking composite materials had uneven distribution but pore size significantly reduced;while glutaraldehyde cross-linking group had regular structure with layered folds

internal pore size became larger and it was mainly dominated by sheet structure.Cross-linked composite groups could facilitate osteoblast proliferation and it was significantly better than the control group and the composite materials group

anxo-action for osteoblast proliferation of glutaraldehyde crosslinking group was better than thermo-crosslinking group.Crosslinking composite materials could effectively improve ALP activity of osteoblast. Conclusion: Glutaraldehyde cross-linking method could effectively improve properties of polypeptides from Cervi Cornu Pantotrichum-collagen/chitosan composite materials

such as osteoblast proliferation promoted and ALP activity increased.