Objective: To optimize extraction process of total alkaloids

total flavonoids and polysaccharides from mulberry seeds. Method: After mulberry seeds was defatted

with composite score of extracting amounts of total alkaloids

total flavonoids and polysaccharides as indicators

on the basis of single factor tests

orthogonal design was adopted to optimize extraction process by taking extracting time

ethanol concentration

hydrochloric acid concentration as factors.Contents of total flavonoids

total alkaloids and polysaccharides were determined by Al (NO3)3

reinecke salt colorimetry and anthrone-sulfuric acid method

respectively. Result: Optimum extraction conditions were as follows:extracting time 2 h

ethanol concentration 50%

molar concentration of hydrochloric acid 0.03 mol·L-1;average extracting amounts of total alkaloids

total flavonoids and polysaccharides were 23.15

0.57

0.51 mg·g-1. Conclusion: This optimized extraction process was stable

feasible and suitable for industrial production with high extraction efficiency

it could provide a reference for development and utilization of mulberry seeds resources.