Objective: To establish the fingerprint chromatograms for the quality evaluation of Qingre Jiedu Koufuye By HPLC. Method: The analysis was performed on a Shim-pack VP-ODS column(4.6 mm×250 mm

5 μm) with acetonitrile-water (containing 0.02%(v/v)phosphatacid) as mobile phase at a flow rate of 1.0 mL·min-1. The detection wave-length was at 270 nm. Mobile phase A is acetonitrile;Mobile phase B for 0.02% phosphoric acid water

mobile phase A gradient elution (0-12 min，1%-15%A;12-22 min

15%-20%A

22-32 min

20%-25%A

32-40 min

25%-45%A;40-50 min

45%-55%A). Result: Twelve batch of Qingre Jiedu Koufuye sample has nine common peak. The analysis results of similarity between 0.911 and 0.972. Conclusion: Qingre Jiedu Koufuye fingerprint of the characteristic and strong specificity

and can be used in the comprehensive control Qingre Jiedu Koufuye quality

ensure every batch of product uniformity.