Objective: To establish the chromatographic fingerprint for Guizhi Gancao decoction by RP-HPLC-DAD. Method: The HPLC fingerprint were determined on Agilent ZORBAX SB-Aq column(4.6 mm×150 mm

5 μm)

eluted with the mobile phase consisted of acetonitrile and 0.1% phosphoric acid in gradient mode;flow rate 0.8 mL·min-1;column temperature 25℃ and detection wavelength was set at 254 nm

injection volume 10 μL.Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(2004A) was used to analysis. Result: Twenty five common peaks were selected as the fingerprint peaks of Ramuli Cinnamomi and Glycyrrhizae. And 4 common peaks were determined by the relactive time of the standard substance. The similarity of 10 batches of Guizhi Gancao decoction were all more than 0.95. Conclusion: The established HPLC fingerprint of Guizhi Gancao decoction has desirable precision

reproducibility

and can provide a basis for quality evaluation of Guizhi Gancao decoction.