SPME-GC-MS法研究竹叶柴胡和北柴胡挥发性成分差异

王砚; 王书林

doi:10.13422/j.cnki.syfjx.2014140104

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SPME-GC-MS法研究竹叶柴胡和北柴胡挥发性成分差异

化学与分析 | 更新时间：2024-01-04

- SPME-GC-MS法研究竹叶柴胡和北柴胡挥发性成分差异
- Different Analysis of Essential Oil from and by SPME-GC-MS
- 中国实验方剂学杂志 2014年20卷第14期页码：104-108
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家星火计划项目（2010GA810056）
- DOI：10.13422/j.cnki.syfjx.2014140104
  中图分类号： O657.63;R284.1
- 纸质出版日期：2014
- 稿件说明：
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王砚, 王书林. SPME-GC-MS法研究竹叶柴胡和北柴胡挥发性成分差异[J]. 中国实验方剂学杂志, 2014,20(14):104-108.

WANG Yan, WANG Shu-lin. Different Analysis of Essential Oil from and by SPME-GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 104-108.
王砚, 王书林. SPME-GC-MS法研究竹叶柴胡和北柴胡挥发性成分差异[J]. 中国实验方剂学杂志, 2014,20(14):104-108. DOI： 10.13422/j.cnki.syfjx.2014140104.

WANG Yan, WANG Shu-lin. Different Analysis of Essential Oil from and by SPME-GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 104-108. DOI： 10.13422/j.cnki.syfjx.2014140104.

摘要

目的：对竹叶柴胡和北柴胡进行挥发性成分的比较和分析，指出竹叶柴胡和北柴胡的差异性和共同性。方法：利用固相微萃取技术（SPME）提取，采用气质联用（GC-MS）进行分离和鉴定。结果：共鉴定出竹叶柴胡48个成分，北柴胡21个成分，其中有10个化合物为共有峰，相对质量分数最高的均为n-十六烷酸。结论： SPME-GC-MS能全面快速分析柴胡挥发油成分，并作为柴胡药材质量评估和基源鉴定的科学手段。

Abstract

Objective: To find the difference and the identity through analyzing the essential oil of Bupleurum marginatum and B. chinese. Method: The essential oil components of B. marginatum and B. chinese were extracted by solid phase micro extraction(SPME) and analyzed by GC-MS. Result: Forty-eight compounds were identified from B. marginatum.and 21 from B. Chinese. Ten common compounds were found and n-hexadecanoic acid was the main compound of highest relative content in both of B. marginatum and B. chinense. Conclusion: The information about the constituents of essential oil can be obtained entirely and quickly by SPME-GC-MS which is the scientific method of quality assessment and origin identification.

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