To make pulmonary fibrosis rat models by Bleomycin

observe pathomorphism changes in these models effects on connective tissue growth factor (CTGF)

intergrin-linked kinase(ILK) in rat model's lungs

and discuss the prevention mechanism of Xianke granules on pulmonary fibrosis. Method: Divide rats into 6 groups at random

which are control group

model group

prednisone group

high dose Xianke group(HD group)

medium dose Xianke group(MD group) and low dose Xianke group(LD group)

with 30 rats in each group. They were fed with common forage and were made into the models of pulmonary fibrosis. the 7th day

14th day

and 28th day respectively

measure Pulmonary fibrosis degrees in the lung dyed with HE and Masson;measure the expression of CTGF

ILK in the lung by immunol histochemistry. Result: There is increased CTGF

ILK expression of lung tissue in model group at all time points

which is significantly higher than that in blank group. There are large number of yellow particles around lung tissue and alveolar wall

and there are dark yellow particles in focal vessels

part of inflammatory cell exuded from bronchus

focus and alveolar septum in interstitial cell

and there is remarkable statistical significance

P<0.01. Compared between Xianke granules HD

MD and LD groups and prednisone group and model group

there is CTGF positive expression in pulmonary interstice and around alveolar wall on day 7

14 and 28

and there is remarkable statistical significance

P<0.05.Compared between Xianke granules HD

MD groups and prednisone group on day 7

there is no remarkable statistical significance;compared between Xianke granules LD groups and prednisone group

and there is remarkable statistical significance

P<0.05. Conclusion: Xianke granules improve the abnormal deposition of cell epimatrix by restraining the CTGF

ILK expression of pulmonary fibrosis rats' lung tissue

which might be one of the mechanism of preventing pulmonary fibrosis with Xianke granules.