温胆汤对精神分裂症模型鼠血清TNF-，IL-6及海马组织Glu活性表达的影响

朱金华; 孙昊鑫; 熊秋迎; 陈乔; 马广强; 叶荷平

doi:10.13422/j.cnki.syfjx.2014140160

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温胆汤对精神分裂症模型鼠血清TNF-，IL-6及海马组织Glu活性表达的影响

药理 | 更新时间：2024-01-04

- 温胆汤对精神分裂症模型鼠血清TNF-，IL-6及海马组织Glu活性表达的影响
- Effect of Wendan Decoction on Contents of Serum TNF-, IL-6 and Expression of Glu in Hippocampus Tissue in Rats with Schizophrenia
- 中国实验方剂学杂志 2014年20卷第14期页码：160-164
- 作者机构：
- 作者简介：
- 基金信息：
  
  江西省卫生厅中医药一般计划（2012A156）;江西中医药大学重点学科青年教师培养计划（2012jzzdxk027）
- DOI：10.13422/j.cnki.syfjx.2014140160
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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朱金华, 孙昊鑫, 熊秋迎, 等. 温胆汤对精神分裂症模型鼠血清TNF-，IL-6及海马组织Glu活性表达的影响[J]. 中国实验方剂学杂志, 2014,20(14):160-164.

ZHU Jin-hua, SUN Hao-xin, XIONG Qiu-ying, et al. Effect of Wendan Decoction on Contents of Serum TNF-, IL-6 and Expression of Glu in Hippocampus Tissue in Rats with Schizophrenia[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 160-164.
朱金华, 孙昊鑫, 熊秋迎, 等. 温胆汤对精神分裂症模型鼠血清TNF-，IL-6及海马组织Glu活性表达的影响[J]. 中国实验方剂学杂志, 2014,20(14):160-164. DOI： 10.13422/j.cnki.syfjx.2014140160.

ZHU Jin-hua, SUN Hao-xin, XIONG Qiu-ying, et al. Effect of Wendan Decoction on Contents of Serum TNF-, IL-6 and Expression of Glu in Hippocampus Tissue in Rats with Schizophrenia[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 160-164. DOI： 10.13422/j.cnki.syfjx.2014140160.

摘要

目的：研究温胆汤对精神分裂症模型鼠血清肿瘤坏死因子（TNF-α）、白介素6（IL-6）及海马组织谷氨酸（Glu）活性表达的影响。方法：将72只清洁级SD大鼠随机分为6组：正常组（A）、模型组（B）、温胆汤高剂量组（C）、温胆汤中剂量组（D）、温胆汤低剂量组（E）、氯氮平组（F），每组12只。在造模前，C，D，E组分别ig温胆汤生药40，20，10 g·kg-1；F组ig氯氮平20 mg·kg-1；A，B组用等量生理盐水ig，1次/d，共21 d。在最后1次给药2 h后，B，C，D，E，F组一次性ip MK801 0.6 mg·kg-1，建立精神分裂症模型，并进行刻板行为学观察；3 d后处死大鼠，取血清和海马组织检测。HE染色光镜下观察海马组织CA1区的病理改变，采用双抗体夹心-酶联免疫吸附（ABC-ELISA）法检测血清中TNF-α，IL-6的含量，免疫组化检测海马组织中Glu活性的表达。结果：与正常组比较，模型组大鼠出现刻板行为；血清TNF-α，IL-6含量明显升高（P<0.05或P<0.01）；海马组织Glu蛋白表达水平显著降低（P<0.05或P<0.01）。与模型组比较，温胆汤各剂量组均不同程度改善了模型鼠的刻板行为和海马CA1区的病理改变，并可明显降低血清TNF-α，IL-6的含量（P<0.05或P<0.01），使海马组织Glu活性的表达也显著升高（P<0.05或P<0.01）。结论：温胆汤通过调节免疫功能、减少炎症因子产生和提高Glu活性的表达来减轻精神分裂症模型鼠海马组织的病理损害。

Abstract

Objective: To study the effect of Wendan decoction on the contents of serum tumor necrosis factor-α(TNF-α)

interleukin-6(IL-6) and the expression of glutamic acid(Glu) in hippocampus tissue in rats with schizophrenia. Method: Seventy-two SD rats were randomly divided six groups:normal group(A)

model group(B)

Wendan decoction high-dosage group(C)

Wendan decoction medium-dosage group(D)

Wendan decoction low-dosage group(E)

and Clozapine group(F)

twelve rats in each group. Before the model was established

the C

E groups were respectively given Wendan decoction (40

10 g·kg-1)

the F group was given Clozapine (20 mg·kg-1)

the A and B groups were given normal saline every day

which last for 21 days. At the last given Wendan decoction or saline after two hours

the B

F groups were ip given MK801 0.6 mg·kg-1 to establish rat schizophrenia model

and stereotyped behavior of rats were observed. After three days

the rats were sacrificed

the serum and the hippocampus tissue were tested. To observe the CA1 region of the hippocampus by microscope and HE staining.The contents of serum TNF-α

IL-6 were detected by double antibody clip ELASA method. The expression of Glu in hippocampus tissue was determined by immunohistochemical method. Result: Compared with A group

rats of B group appeared stereotyped behavior

the contents of serum TNF-α

IL-6 were significantly increased (P<0.05 or P<0.01)and the expression of Glu in hippocampus tissue was decreased(P<0.05 or P<0.01)in rats of B group.Compared with B group

stereotyped behavior and pathological change of the CA1 region in hippocampus tissue were improved

Wendan decoction could significantly decrease the contents of serum TNF-α

IL-6(P<0.05 or P<0.01)and increase the expression of Glu in hippocampus tissue(P<0.05 or P<0.01). Conclusion: Wendan decoction could reduce the pathologic lesions of hippocampus tissue in rat with schizophrenia through regulating immune function

decreasing content of the inflammatory factor and increasing the expression of Glu.

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