黄芩苷壳聚糖纳米粒的制备及表征

吴瑛; 李阳; 李凤琴; 杨华生; 王芳

doi:10.13422/j.cnki.syfjx.2014150015

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黄芩苷壳聚糖纳米粒的制备及表征

工艺与制剂 | 更新时间：2024-01-04

- 黄芩苷壳聚糖纳米粒的制备及表征
- Preparation and Characterization of Baicalin-chitosan Nanoparticles
- 中国实验方剂学杂志 2014年20卷第15期页码：15-19
- 作者机构：
- 作者简介：
- 基金信息：
  
  江西省自然科学基金项目（20114BAB215042）;江西省教育厅青年基金项目（GJJ12533）;江西省卫生厅中医药科研计划项目（2010A091）
- DOI：10.13422/j.cnki.syfjx.2014150015
  中图分类号： R283.6
- 纸质出版日期：2014
- 稿件说明：
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吴瑛, 李阳, 李凤琴, 等. 黄芩苷壳聚糖纳米粒的制备及表征[J]. 中国实验方剂学杂志, 2014,20(15):15-19.

WU Ying, LI Yang, LI Feng-qin, et al. Preparation and Characterization of Baicalin-chitosan Nanoparticles[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 15-19.
吴瑛, 李阳, 李凤琴, 等. 黄芩苷壳聚糖纳米粒的制备及表征[J]. 中国实验方剂学杂志, 2014,20(15):15-19. DOI： 10.13422/j.cnki.syfjx.2014150015.

WU Ying, LI Yang, LI Feng-qin, et al. Preparation and Characterization of Baicalin-chitosan Nanoparticles[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 15-19. DOI： 10.13422/j.cnki.syfjx.2014150015.

摘要

目的：制备和评价黄芩苷壳聚糖纳米粒（BS-CS-NPs）。方法：采用离子交联法制备BS-CS-NPs，以包封率和粒径为评价指标，通过单因素试验考察搅拌时间、转速、壳聚糖（CS）溶液pH、CS-黄芩苷（BC）投料比、CS-多聚磷酸钠（TPP）质量浓度比对制备工艺的影响；以载药量和包封率的综合评分为指标，通过正交试验考察CS-BC投料质量比、CS-TPP质量浓度比及CS溶液pH对制备工艺的影响；采用电子显微镜观察 BC-CS-NPs 的形态，激光粒度分析仪测定粒径大小和Zeta电位，HPLC检测包封率和载药量，检测波长280 nm，流动相甲醇-0.2%磷酸（47：53）。结果：最佳制备工艺为CS-TPP质量浓度比5：1，CS溶液pH 4，CS-BC投料质量比2：1，搅拌时间15 min，转速1 000 r·min-1；BC-CS-NPs外观圆整，粒度分布均匀，平均粒径（314.32±12.36）nm，包封率（54.73±0.26）%，载药量（23.68±0.32）%，多分散系数0.335±0.041，Zeta电位（24.58±0.36）mV。结论：优化的制备工艺稳定可行，BS-CS-NPs形态为均一的类球形实体粒子，形状规则，形态饱满。

Abstract

Objective: To prepare and assess of baicalin-chitosan nanoparticles(BC-CS-NPs). Method: BC-CS-NPs were prepared by ionic cross-linking.Single factor experiments were used to inspect effects regularity of stirring time

roration rate

pH of chitosan(CS) solution

feeding ratio of CS-baicalin(BC) and concentration ratio of CS-sodium tripolyphosphate(TPP) on encapsulation efficiency and particle size;With composite score of drug loading and encapsulation efficiency as index

orthogonal test was adopted to optimize preparation technology of BC-CS-NPs by taking pH of CS solution

feeding ratio of CS-BC and concentration ratio of CS-TPP as factors;morphology of BC-CS-NPs was observed by electron microscope

particle size and Zeta potential were determined by laser particle size analyzer

HPLC was employed to determine drug loading and encapsulation efficiency with mobile phase of methanol-0.2% phosphoric acid(47:53) and detection wavelength at 280 nm. Result: Optimum preparation conditions were as follows:stirring time of 15 min

roration rate of 1000 r·min-1

pH of CS solution 4

CS-BC(2:1) and CS-TPP(5:1);these prepared BC-CS-NPs had round appearance and uniform particle size distribution

mean diameter of BC-CS-NPs was (314.32±12.36)nm

entrapment efficiency and drug-loading efficiency were (54.73±0.26)% and (23.68±0.32)%

polydispersity was 0.335±0.041

Zeta potential was (24.58±0.36) mV. Conclusion: This optimized preparation process was stable and feasible

BC-CS-NPs showed uniform spherical solid particles with regular shape and full form.

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