Objective: To optimize ethanol extraction process of Bushen Huoxue granules. Method: HPLC was adopted to determine contents of cinnamaldehyde and amygdalin with mobile phase of acetonitrile(A)-water(B) for gradient elution(0-3.5 min

18%A;3.5-4.0 min

18%-55%A;4.0-9.0 min

55%A;9.0-9.5 min

55%-18%A)

detection wavelength at 290 and 210 nm

respectively.Taking transfer rates of cinnamaldehyde and amygdalin as a comprehensive evaluation index

effects of extracting method

soaking time and ethanol concentration on ethanol extraction process were investigated by single factor tests

orthogonal test was employed to optimize extraction process with ethanol consumption

extraction times and extracting time as factors. Result: Optimum extraction process was as follows:soaked 1 h with 10 times the amount of 60% ethanol

extracted thrice for 1.5 h each time;transfer rates of cinnamaldehyde and amygdalin were 85.43% and 86.95%

respectively. Conclusion: This optimized extraction technology was repeatable and feasible

it could provide a reference for industrial production of Bushen Huoxue granules.