DEAE纤维素纯化玉米须总多糖的工艺优选

孙晓雪; 康杰; 王昶; 王伟明

doi:10.13422/j.cnki.syfjx.2014150028

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DEAE纤维素纯化玉米须总多糖的工艺优选

工艺与制剂 | 更新时间：2024-01-04

- DEAE纤维素纯化玉米须总多糖的工艺优选
- Optimization of Purification Process for Total Polysaccharides in Stigma Maydis with DEAE Cellulose
- 中国实验方剂学杂志 2014年20卷第15期页码：28-30
- 作者机构：
- 作者简介：
- 基金信息：
  
  黑龙江省科技攻关项目（GA12C102）
- DOI：10.13422/j.cnki.syfjx.2014150028
  中图分类号： R284
- 纸质出版日期：2014
- 稿件说明：
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孙晓雪, 康杰, 王昶, 等. DEAE纤维素纯化玉米须总多糖的工艺优选[J]. 中国实验方剂学杂志, 2014,20(15):28-30.

SUN Xiao-xue, KANG Jie, WANG Chang, et al. Optimization of Purification Process for Total Polysaccharides in Stigma Maydis with DEAE Cellulose[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 28-30.
孙晓雪, 康杰, 王昶, 等. DEAE纤维素纯化玉米须总多糖的工艺优选[J]. 中国实验方剂学杂志, 2014,20(15):28-30. DOI： 10.13422/j.cnki.syfjx.2014150028.

SUN Xiao-xue, KANG Jie, WANG Chang, et al. Optimization of Purification Process for Total Polysaccharides in Stigma Maydis with DEAE Cellulose[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 28-30. DOI： 10.13422/j.cnki.syfjx.2014150028.

摘要

目的：优选玉米总多糖的DEAE纤维素纯化工艺，确定中性多糖和酸性多糖的比例。方法：采用蒽酮-浓硫酸法测定总多糖含量，检测波长625 nm。通过单因素试验考察上样量、洗脱剂浓度及用量对DEAE纤维素纯化玉米须总多糖的影响。结果：最佳纯化工艺为上样量0.75 BV，分别用10 BV水和15 BV的0.1 mol·L-1 NaCl溶液洗脱，可较充分地将中性总多糖和酸性总多糖洗脱下来；中性多糖和酸性多糖分别占总多糖的30.43%和58.88%，纯度分别提高了约2.5和2倍。结论：DEAE纤维素纯化玉米须总多糖效果较好，该法适合玉米须总多糖的纯化，可分离中性总多糖和酸性总多糖。

Abstract

Objective: To optimize purification process of total polysaccharides from Stigma Maydis by DEAE cellulose and determine proportion of neutral and acidic polysaccharides. Method: Anthrone sulfuric acid method was adopted to determine the content of total polysaccharides with detection wavelength at 625 nm.Single factor tests were used to investigate effects of sample amount

concentration and dosage of eluant on purifition process of total polysaccharides from Stigma Maydis by DEAE cellulose. Result: The best purification process was as following:sample volume of 0.75 BV

eluted with 10 BV of water and 15 BV of 0.1 mol·L-1 NaCl solution;concentrations of neutral and acidic polysaccharides in total polysaccharides were 30.43% and 58.88%

purities of them increased about 2.5 and 2 times

respectively. Conclusion: Purification of total polysaccharides from Stigma Maydis with DEAE cellulose was suitable and it could separate neutral polysaccharides and acid polysaccharides at the same time.

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