响应面法优化秦岭龙胆有效成分的提取工艺

许海燕; 郑伶俐; 多本加; 刘瑞林

doi:10.13422/j.cnki.syfjx.2014150031

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响应面法优化秦岭龙胆有效成分的提取工艺

工艺与制剂 | 更新时间：2024-01-04

- 响应面法优化秦岭龙胆有效成分的提取工艺
- Optimization of Extraction Process for Active Ingredients in by Response Surface Methodology
- 中国实验方剂学杂志 2014年20卷第15期页码：31-34
- 作者机构：
- 作者简介：
- 基金信息：
  
  陕西省教育厅项目（2013JK0804）
- DOI：10.13422/j.cnki.syfjx.2014150031
  中图分类号： R284
- 纸质出版日期：2014
- 稿件说明：
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许海燕, 郑伶俐, 多本加, 等. 响应面法优化秦岭龙胆有效成分的提取工艺[J]. 中国实验方剂学杂志, 2014,20(15):31-34.

XU Hai-yan, ZHENG Ling-li, DUO Ben-jia, et al. Optimization of Extraction Process for Active Ingredients in by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 31-34.
许海燕, 郑伶俐, 多本加, 等. 响应面法优化秦岭龙胆有效成分的提取工艺[J]. 中国实验方剂学杂志, 2014,20(15):31-34. DOI： 10.13422/j.cnki.syfjx.2014150031.

XU Hai-yan, ZHENG Ling-li, DUO Ben-jia, et al. Optimization of Extraction Process for Active Ingredients in by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 31-34. DOI： 10.13422/j.cnki.syfjx.2014150031.

摘要

目的：优选秦岭龙胆中齐墩果酸的乙醇回流提取工艺。方法：采用HPLC测定齐墩果酸含量，流动相乙腈-甲醇-水-磷酸-三乙胺（25：15：10：0.01：0.02），检测波长210 nm。在单因素试验基础上，根据Box-Benhnken中心组合设计原理，利用三因素三水平响应面分析法考察料液比、提取次数、提取时间对齐墩果酸提取量的影响。结果：最佳提取工艺为料液比1：12，提取数2次，提取时间76 min；齐墩果酸提取量0.583 mg·g-1，与预测值0.605 mg·g-1的偏差仅3.64%。结论：采用响应面法优选的秦岭龙胆回流提取工艺稳定可行，为秦岭龙胆的资源开发及临床推广提供参考。

Abstract

Objective: To optimize ethanol reflux extraction process of oleanolic acid in Gentiana apiata. Method: HPLC was employed to determine the content of oleanolic acid with mobile phase of acetonitrile-methanol-water-phosphoric acid-triethylamine(25:15:10:0.01:0.02) and detection wavelength at 210 nm.On the basis of single factor tests

according to Box-Behnken design principles

response surface analysis was adopted to investigate effects of solid-to-liquid ratio

extraction times and extracting time on yield of oleanolic acid. Result: Optimal extraction conditions were as follows:solid-to-liquid ratio of 1:12

extracted twice

extracting time of 76 min;under these conditions

yield of oleanolic acid was 0.583 mg·g-1

closed to predicted yield of 0.605 mg·g-1. Conclusion: This optimized process was feasible and stable

it had a certain reference value for resource development and clinical promotion of G. apiata.

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