Objective: To optimize materials compatibility in preparation process of Massa Medicata Fermentata. Method: Taking wheat bran-flour and ratio of wheat bran and wheat flour to Vignae Semen as factors

Massa Medicata Fermentata with different materials compatibility fermented5days in constant temperature and humidity box

then took out

after low temperature drying

enzyme liquid was prepared

enzyme activities of amylase

saccharifying enzyme and protease were determined

select the best ingredients proportion according to these three indicators

its methanol extract was analyzed by HPLC fingerprint

taking specific chromatogram spectrum of Massa Medicata Fermentata with the best ingredients proportion as reference spectrum

similarity was evaluated by 2004 A version of ‘fingerprint similarity evaluation system of traditional Chinese medicine’. Result: The best wheat bran-flour ratio was 70:30

optimum ratio of carbon and nitrogen was 100:2.Fingerprint similarities of different samples were more than 0.9. Conclusion: Chemical composition in Massa Medicata Fermentata with high enzyme activity had no significant differences by comparing with other samples

it was suitable for practical application.