Objective: A high-performance liquid chromatographic method was established to evaluate the quality of Sorbus tianschanica with a simultaneous determination of six compounds[rutin

hyperoside

isoquercitrin

quercetin-3-O-(6"-O-malonyl)-β-D-glucoside

kaempferol-3-O-β-D-glucopyranoside and kaempferol-3-O-(6"-O-malonyl)-β-D-glucpyranoside]. Method: The Diamonsil C18(4.6 mm ×250 mm

5 μm) column was adopted and diluted with acetonitrile-0.4%potassium dihydrogen phosphate gradiently at the wavelength of 360 nm. The flow rate was 1.0 mL·min-1 and the column temperature was 25℃. Result: The method had good linearity over the range of 3.3-39.5

6.2-74.1

12.3-147.5

14.3-171.2

7.5-90.4

15.6-187.5 mg·L-1 for rutin

hyperoside

isoquercitrin

quercetin-3-O-(6"-O-malonyl)-β-D-glucoside

kaempferol-3-O-β-D-glucopyranoside

kaempferol-3-O-(6"-O-malonyl)-β-D-glucopyranoside

respectively.The average recovery (n=6) of six components were 98.1%

97.5%

94.5%

95.8%

102.2%

and 97.9%

with RSDs of 11.37%

1.57%

1.02%

1.77%

2.48%

and 1.38% (n=6). Conclusion: The developed method is convenient

rapid and accurate with high repeatability and stability

it is suitable for the content determination.