Objective: To establish a capillary GC method for the determination of campesterol

stigmasterol and β-sitosterol in feedstuff. Method: Agilent DB-1701 column (0.25 μm× 0.32 mm×30 m) was adopted. The inlet temperature was controlled at 280℃ and the FID detector temperature was 300℃. The oven temperature was initially held at 100℃ for 2 min and was then programmed to 270℃ at 20℃·min-1 and held for another 15 min. Result: A good resolution was obtained between the peaks of campesterol

stigmasterol and β-sitosterol. The linearity range was 51.08-255.4

20.86-104.3

16.13-80.64 mg·L-1respectively

the sample average recovery was 88.5%-98.4%. RSD was range from 0.23% to 3.27%. Conclusion: The method has the advantages of short peak retention

high sensitivity

accurate

reliable

good reproducibility and selectivity

and can contribute to the improvement of feedstuff quality standard by providing scientific data.