HPLC-FLD免疫亲和柱-光衍生化法测定胖大海中黄曲霉毒素的残留量

苏静华; 张超; 钟水生; 汪明志

doi:10.13422/j.cnki.syfjx.2014150075

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HPLC-FLD免疫亲和柱-光衍生化法测定胖大海中黄曲霉毒素的残留量

化学与分析 | 更新时间：2024-01-04

- HPLC-FLD免疫亲和柱-光衍生化法测定胖大海中黄曲霉毒素的残留量
- Quantitative Analysis of Aflatoxin in Sterculiae Lychnophorae Semen by HPLC-FLD after Immunoaffinity Column with Post-column Photochemical Derivatization
- 中国实验方剂学杂志 2014年20卷第15期页码：75-78
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西科技开发项目（桂科攻11107010-2-1）;广西卫生厅中医药科技专项（GZPT1228）;广西重大专项计划（桂科重1355001-4）
- DOI：10.13422/j.cnki.syfjx.2014150075
  中图分类号： O657.72;R284.1
- 纸质出版日期：2014
- 稿件说明：
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苏静华, 张超, 钟水生, 等. HPLC-FLD免疫亲和柱-光衍生化法测定胖大海中黄曲霉毒素的残留量[J]. 中国实验方剂学杂志, 2014,20(15):75-78.

SU Jing-hua, ZHANG Chao, ZHONG Shui-sheng, et al. Quantitative Analysis of Aflatoxin in Sterculiae Lychnophorae Semen by HPLC-FLD after Immunoaffinity Column with Post-column Photochemical Derivatization[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 75-78.
苏静华, 张超, 钟水生, 等. HPLC-FLD免疫亲和柱-光衍生化法测定胖大海中黄曲霉毒素的残留量[J]. 中国实验方剂学杂志, 2014,20(15):75-78. DOI： 10.13422/j.cnki.syfjx.2014150075.

SU Jing-hua, ZHANG Chao, ZHONG Shui-sheng, et al. Quantitative Analysis of Aflatoxin in Sterculiae Lychnophorae Semen by HPLC-FLD after Immunoaffinity Column with Post-column Photochemical Derivatization[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 75-78. DOI： 10.13422/j.cnki.syfjx.2014150075.

摘要

目的：利用HPLC-FLD结合免疫亲和柱-光衍生化法测定胖大海药材中黄曲霉毒素残留量。方法：采用Cloversil C18 色谱柱，流动相甲醇-水（47：53），等度洗脱，流速1 mL·min-1，柱温35℃，在此条件下进行测定。结果：黄曲霉毒素的检测限和定量限均低于0.2 μg·L-1，平均回收率97.06%~99.93%，RSD 低于 12%，精密度、重复性均达到痕量分析的要求。结论：该方法结果可靠，可用于胖大海中测定黄曲霉毒素 B1， B2， G1， G2含量的要求。

Abstract

Objective: To establish a stable method for quantitative analysis of aflatoxin in Sterculiae Lychnophorae Semen by HPLC-FLD after immunoaffinity column with post-column photochemical derivatization. Method: Cloversil C18 column was applied and mobile phase was consisted of methanol and water (47:53) with gradient elution of 1 mL·min-1

column temperature was maintained at 35℃. Result: The detection limits and quantitation limits of aflatoxin were lower than 0.2 μg·L-1

average recoveries were in range of 97.06%-99.93%

and RSDs were below 12%. Besides that

precisions and repetitiveness all achieve the requirement. Conclusion: The method is a simple

accurate and could be used to analyze the content of aflatoxin in Sterculiae Lychnophorae Semen.

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