Objective: To select the perfect method of extracting technology of total flavonoid from Kaifeng Ornamental chrysanthemum and to find differences of content from different parts. Method: Using total flaconoids content as indicator

firstly studying and selecting the the optimum extraction technology from microwave and ultrasonic by extraction time

solid-liquidratio and ethanol concentration. Then the total flaconoids content of Ornamental Kaifeng Chrysanthemum Dali flowers

leaves

stems were extracted by the optimum extraction technology and determined with UV-Vis spectrophotometry. Result: The optimized extraction technology of ultrasonic method was extraction time 30 min

solid-liquid ratio 1:25 and ethanol concentration 80%. The total flavonoids content showed a linear range of 20-70 mg·L-1

the average recoveries of the total flaconoids content was 97.5%

RSD was 1.81%. Among the three kinds of chrysanthemum

Purple Chrysanthemum cliff was the highest content of total flavonoids and Dali Chrysanthemum was the lowest. The content of total flavonoids was the highest in Dali Chrysanthemum flowers

second in leaves

and the lowest in stems. Conclution: The method of ultrasonic is high efficiency and stability

which can be the best method of extracting technology of total flavonoid from Ornamental Chrysanthemum

the UV-Vis is very simple

quick

accurate

reliable and stability

which can provide more reference for studying the quality control of Ornamental Chrysanthemum.