铁皮石斛对运动疲劳小鼠能量代谢和CNTFmRNA表达的影响

唐汉庆; 陈桦; 韦祎; 卢兰

doi:10.13422/j.cnki.syfjx.2014150164

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铁皮石斛对运动疲劳小鼠能量代谢和CNTFmRNA表达的影响

药理 | 更新时间：2024-01-04

- 铁皮石斛对运动疲劳小鼠能量代谢和CNTFmRNA表达的影响
- Effects of on Energy Metabolism and Expression of CNTF mRNA in Athletic Fatigue Mice
- 中国实验方剂学杂志 2014年20卷第15期页码：164-167
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西教育厅科研立项（201106LX443）
- DOI：10.13422/j.cnki.syfjx.2014150164
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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唐汉庆, 陈桦, 韦祎, 等. 铁皮石斛对运动疲劳小鼠能量代谢和CNTFmRNA表达的影响[J]. 中国实验方剂学杂志, 2014,20(15):164-167.

TANG Han-qing, CHEN Hua, WEI Yi, et al. Effects of on Energy Metabolism and Expression of CNTF mRNA in Athletic Fatigue Mice[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 164-167.
唐汉庆, 陈桦, 韦祎, 等. 铁皮石斛对运动疲劳小鼠能量代谢和CNTFmRNA表达的影响[J]. 中国实验方剂学杂志, 2014,20(15):164-167. DOI： 10.13422/j.cnki.syfjx.2014150164.

TANG Han-qing, CHEN Hua, WEI Yi, et al. Effects of on Energy Metabolism and Expression of CNTF mRNA in Athletic Fatigue Mice[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 164-167. DOI： 10.13422/j.cnki.syfjx.2014150164.

摘要

目的：观察铁皮石斛对运动疲劳小鼠能量代谢和睫状神经营养因子（ciliary neurotrophic factor，CNTF） mRNA表达的影响。方法：清洁级雄性昆明种小鼠60只随机分为正常对照组（简称对照组）、铁皮石斛低、中、高剂量组共4组。每组15只。对铁皮石斛低、中、高剂量组按0.75，1.5，4.5 g·kg-1分别给予铁皮石斛灌胃，对照组灌胃相同体积的生理盐水。每天1次，连续30 d。然后通过负重游泳实验，观察小鼠负重游泳时间，检测血清尿素、肌糖原、肝糖原及乳酸含量和CNTF mRNA表达。结果：与对照组负重游泳时间比较，铁皮石斛低、中、高剂量组均显著延长（P<0.05或P<0.01）。与对照组血清尿素、乳酸含量比较，铁皮石斛低、中、高剂量组均显著降低（P<0.05或P<0.01）。与对照组肝糖原、肌糖原含量比较，铁皮石斛中、高剂量组均显著升高（P<0.05或P<0.01）。与对照组CNTF mRNA表达量比较，铁皮石斛低、中、高剂量组均显著升高（P<0.05或P<0.01）。结论：铁皮石斛通过提高机体糖原储备、减少血清尿素含量、减轻乳酸堆积，同时提高CNTF mRNA表达，从调节能量代谢和营养肌肉两方面发挥功效可能是其抗疲劳的机制之一。

Abstract

Objective: To investigate the effects of Dendrobium officinale on energy metabolism and expression of ciliary neurotrophic factor (CNTF) mRNA in athletic fatigue mice. Method: Sixty male Kunming mice were divided randomly into control group

D. officinale low-dose group

middle-dose group

high-dose group. The low-dose group

middle-dose group and high-dose group were intragastricaly (ig)administrated with D. officinale respectively according to 0.75

1.5

4.5 g·kg-1 while control group was ig given same volume normal saline

once a day for 30 days. The weight carrying swimming test were used to observe exhaustive weight carrying swimming time and to examine levels of serum urea

muscle glycogen

hepatic glycogen

lactic acid and the expression of CNTF mRNA. Result: Compared to control group

exhaustive weight carrying swimming time in low-dose group increased (P<0.05)

and in both middle-dose group and high-dose group increased significantly(P<0.01). Compared to control group

the levels of serum urea and lactic acid in both low-dose group and middle-dose group decreased(P<0.05)

and in high-dose group decreased significantly(P<0.01). Compared to control group

the levels of muscle glycogen and hepatic glycogen in middle-dose group increased (P<0.05)and in high-dose group increased significantly (P<0.01). Compared to control group

the expression of CNTF mRNA in low-dose group increased (P<0.05)

and in middle-dose group and high-dose group increased significantly(P<0.01). Conclusion: D. officinale could increase glycogen store

decrease the level of serum urea and lactic acid accumulation

meantime

could up-regulate the expression of CNTF mRNA

which may be one of mechanisms that D.officinale alleviate athletic fatigue by regulating energy metabolism and nourishing muscle.

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