Objective: To evaluate the acute and mutagenic toxic effects of the active ingredients assembling of Chinese herbs for antioxidant activity. Chinese herbal active ingredient formula was composed of total flavonoids of Glycyrrhiza

ginkgo biloba extract

total flavonoids of epimedium

astragaloside and total saponins in ophiopogonin. Method: Acute oral toxicity test

marrow cell micronucleus test polychromatic erythrocyte(PCE)

marrow chromosomal aberration test of mice and Ames were tested. Result: Mice acute oral maximum tolerated dose(MTD) of the formula was higher than 10 g·kg-1. The micronucleus rates in all doses were not significantly different from the control group. Negative results were seen form chromosome aberration test. No increase in the number of revertant colonies was found in the Ames test with and without S9 activation system. Conclusion: The Chinese herbal active ingredient formula has no toxic substance and mutagenicity effects in this study.