Box-Behnken响应面法优选金樱子抗糖尿病活性部位提取工艺

柴思佳; 齐海艳; 邓翀

doi:10.13422/j.cnki.syfjx.2014160026

您当前的位置：

首页 >

文章列表页 >

Box-Behnken响应面法优选金樱子抗糖尿病活性部位提取工艺

工艺与制剂 | 更新时间：2024-01-04

- Box-Behnken响应面法优选金樱子抗糖尿病活性部位提取工艺
- Optimization of Extraction Process for Anti-diabetic Active Parts from Rosae Laevigatae Fructus by Box-Behnken Response Surface Methodology
- 中国实验方剂学杂志 2014年20卷第16期页码：26-29
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81374028，30902002，81273750）;陕西省中医药管理局项目（13-ZY014）;陕西省重点科技创新团队计划项目（2013KCT-26）
- DOI：10.13422/j.cnki.syfjx.2014160026
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
柴思佳, 齐海艳, 邓翀. Box-Behnken响应面法优选金樱子抗糖尿病活性部位提取工艺[J]. 中国实验方剂学杂志, 2014,20(16):26-29.

CHAI Si-jia, QI Hai-yan, DENG Chong. Optimization of Extraction Process for Anti-diabetic Active Parts from Rosae Laevigatae Fructus by Box-Behnken Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 26-29.
柴思佳, 齐海艳, 邓翀. Box-Behnken响应面法优选金樱子抗糖尿病活性部位提取工艺[J]. 中国实验方剂学杂志, 2014,20(16):26-29. DOI： 10.13422/j.cnki.syfjx.2014160026.

CHAI Si-jia, QI Hai-yan, DENG Chong. Optimization of Extraction Process for Anti-diabetic Active Parts from Rosae Laevigatae Fructus by Box-Behnken Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 26-29. DOI： 10.13422/j.cnki.syfjx.2014160026.

摘要

目的：优选金樱子抗糖尿病活性部位的提取工艺。方法：以体外抗α-淀粉酶活性和抗氧化活性的综合评分为因变量，乙醇体积分数、料液比、提取时间、提取次数为自变量，采用Design Expert 8.05软件分析试验数据，建立多元二次回归方程，采用Box-Behnken响应面法优选金樱子抗糖尿病活性部位的提取工艺。结果：最佳提取工艺为加11倍量85% 乙醇提取2次，每次120 min；α-淀粉酶活性平均抑制率95.39%，RSD 4.59%，DPPH自由基平均清除率91.47%，RSD 4.12%，综合评分0.967 2，与预测值0.977 8吻合程度良好。结论：通过生物活性评价优选的提取工艺更科学合理，符合临床实际应用。

Abstract

Objective: To optimize extraction process of anti-diabetic active parts from Rosae Laevigatae Fructus. Method: With composite score of in vitro anti-α-amylase and anti-oxidation activities as dependent variable

ethanol concentration

solid-liquid ratio

extraction time and times as independent variables

test data were analyzed by Design Expert 8.05 software

then multivariate quadratic regression equation was established

Box-Behnken response surface methodology was adopted to optimize extraction process of anti-diabetic active parts from Rosae Laevigatae Fructus. Result: Optimal process parameters were as follows:extracted twice with 11 times the amount of 85% ethanol for 120 min per time;average inhibition rate of α-amylase was 95.39% with RSD of 4.59%

average clearance rate of DPPH was 91.47% with RSD of 4.12%

composite score was 0.967 2

which was fit with its predictive value of 0.977 8. Conclusion: Optimization of extraction process was scientific and conformed with clinical practical application by biological activity evaluation.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

辣木叶标准汤剂的煎煮工艺优化及质量标准建立

响应面法优化黄芩中黄芩苷的电磁裂解提取工艺

正交试验优选无糖外感风痧颗粒的成型工艺

响应面法优化贯叶连翘中金丝桃素超声提取工艺