Objective: To optimize purification process of total tannins from Juglans mandshurica leaf by macroporous resin. Method: Adsorption and elution capacities of AB-8

D101

NKA-9

Daion HP-20 macroporous resin were investigated by static adsorption-elution tests

single factor tests were adopted to optimize purification process of total tannins from J. mandshurica leaf with sample volume

water amount

elution solvent as factors.The content of total tannins was measured by casein

detection wavelength was set at 760 nm. Result: AB-8 macroporous resin was selected

its optimal purification technology was as follows:the concentration of sample solution 2.672 g· L-1

sample volume of 250 mL

elution rate of 2 BV· h-1

washed impurity with 6 BV of water

collected 10 BV of 50% ethanol eluent;elution rate and purity of total tannins were 73.32% and 35.02%

respectively. Conclusion: AB-8 macroporous resin had goog separation performance for total tannin from J. mandshurica leaf

this optimized purification process was suitable for industrial production.