多指标正交试验法优化肝友胶囊的提取工艺

邓礼荷; 梁结斐; 谭雄斯

doi:10.13422/j.cnki.syfjx.2014160039

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多指标正交试验法优化肝友胶囊的提取工艺

工艺与制剂 | 更新时间：2024-01-04

- 多指标正交试验法优化肝友胶囊的提取工艺
- Optimization of Extraction Technology of Ganyou Capsules by Multi-index Orthogonal Test
- 中国实验方剂学杂志 2014年20卷第16期页码：39-41
- 作者机构：
- 作者简介：
- 基金信息：
  
  肇庆市科技创新项目（2013E126）
- DOI：10.13422/j.cnki.syfjx.2014160039
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
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邓礼荷, 梁结斐, 谭雄斯. 多指标正交试验法优化肝友胶囊的提取工艺[J]. 中国实验方剂学杂志, 2014,20(16):39-41.

DENG Li-he, LIANG Jie-fei, TAN Xiong-si. Optimization of Extraction Technology of Ganyou Capsules by Multi-index Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 39-41.
邓礼荷, 梁结斐, 谭雄斯. 多指标正交试验法优化肝友胶囊的提取工艺[J]. 中国实验方剂学杂志, 2014,20(16):39-41. DOI： 10.13422/j.cnki.syfjx.2014160039.

DENG Li-he, LIANG Jie-fei, TAN Xiong-si. Optimization of Extraction Technology of Ganyou Capsules by Multi-index Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 39-41. DOI： 10.13422/j.cnki.syfjx.2014160039.

摘要

目的：优化肝友胶囊中火炭母等14味饮片的提取工艺。方法：以虎杖苷和白藜芦醇提取量的综合评分为指标，通过正交试验考察加水量、提取时间、提取次数和浸泡时间对肝友胶囊提取工艺的影响。采用HPLC测定虎杖苷和白藜芦醇含量，流动相甲醇-0.5% 磷酸水溶液（35：65），检测波长306 nm，流速0.9 mL·min-1。结果：最佳提取工艺条件为加8倍量水浸泡0.5 h，提取2次，每次2.0 h；虎杖苷、白藜芦醇平均提取量分别为0.676，0.198 mg·g-1，RSD依次为0.56%，0.91%。结论：该工艺稳定可行，为提高肝友胶囊的质量稳定性和临床用药安全提供实验依据。

Abstract

Objective: To optimize extraction technology of Herba Polygoni Chinensis and other Thirteen kinds of medicinal materials in Ganyou capsules. Method: With composite score of extraction amounts of polydatin and resveratrol as index

orthogonal test was adopted to optimize water extraction technology of Ganyou capsules by taking the amount of water

soaking time

extraction time and times as factors.Contents of polydatin and resveratrol were determined by HPLC

mobile phase consisted of methanol-0.5% phosphoric acid solution (35:65)

detection wavelength was set at 306 nm and flow rate was 0.9 mL· min-1. Result: Optimum extraction conditions were as follows:soaked 0.5 h with eight times the amount of water

extracted twice for 2 h per time;under these conditions

average extracting amounts of polydatin and resveratrol were 0.676

0.198 mg· g-1

RSD were 0.56% and 0.91%

respectively. Conclusion: This optimized extmction technology was stable and feasible

it would provide experimental basis for improvement of quality stability and clinical drug safety of Ganyou capsules.

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