正交试验优选宣木瓜总皂苷的提取工艺

秦海军; 张毅; 马玲; 陈师农; 任燕茹

doi:10.13422/j.cnki.syfjx.2014160045

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正交试验优选宣木瓜总皂苷的提取工艺

工艺与制剂 | 更新时间：2024-01-04

- 正交试验优选宣木瓜总皂苷的提取工艺
- Optimization of Extraction Process for Total Saponins from Chaenomelis Fructus by Orthogonal Test
- 中国实验方剂学杂志 2014年20卷第16期页码：45-47
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家“十二五”科技支撑计划项目（2011BAI04B06）
- DOI：10.13422/j.cnki.syfjx.2014160045
  中图分类号： R284
- 纸质出版日期：2014
- 稿件说明：
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秦海军, 张毅, 马玲, 等. 正交试验优选宣木瓜总皂苷的提取工艺[J]. 中国实验方剂学杂志, 2014,20(16):45-47.

QIN Hai-jun, ZHANG Yi, MA Ling, et al. Optimization of Extraction Process for Total Saponins from Chaenomelis Fructus by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 45-47.
秦海军, 张毅, 马玲, 等. 正交试验优选宣木瓜总皂苷的提取工艺[J]. 中国实验方剂学杂志, 2014,20(16):45-47. DOI： 10.13422/j.cnki.syfjx.2014160045.

QIN Hai-jun, ZHANG Yi, MA Ling, et al. Optimization of Extraction Process for Total Saponins from Chaenomelis Fructus by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 45-47. DOI： 10.13422/j.cnki.syfjx.2014160045.

摘要

目的：优选宣木瓜总皂苷的提取工艺。方法：以宣木瓜总皂苷得率为评价指标，通过单因素试验筛选提取方法，利用正交试验考察乙醇体积分数、提取温度、料液比及提取时间对宣木瓜总皂苷提取工艺的影响。采用UV测定总皂苷含量，检测波长550 nm。结果：最佳乙醇提取工艺为加20倍量80% 乙醇于90℃提取3 h；宣木瓜总皂苷得率1.69%。结论：优选的提取工艺稳定可行，为宣木瓜系列产品的开发提供参考。

Abstract

Objective: To optimize extraction process of total saponins from Chaenomelis Fructus. Method: Taking yield of total saponins as index

extraction method was selected by single factor test

orthogonal test was adopted to optimize extraction process with ethanol concentration

extracting temperature

ratio of material to liquid and extraction time as factors.UV was employed to determine the content of total saponins with detection wavelength at 550 nm. Result: The best extraction process was as following:extracted 3 h with 20 times the amount of 80% ethanol at 90℃;yield of total saponins from Chaenomelis Fructus was 1.69%. Conclusion: This process was stable and feasible

it could provide a reference for development of series products of Chaenomelis Fructus.

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