Objective: This study investigated the changes of chemical ingredient between the crude and processed Psoralea Fructus to provide scientific evidence for elucidating the reason that the toxicity of extremely dry Psoralea Fructus was alleviated after salt-processed. Method: Ultra-performance liquid chromatography method (UPLC) coupled with quadrupole time of flight mass spectrometry (Q-TOF/MS) was used for the analysis of the crude and the processed Psoralea Fructus. And the results were analyzed by Markerlynx software Result: There were significant differences between the crude and processed Psoralea Fructus. The contents of psoralen

isopsoralen

bavachin

corylin

isobavachalcone

bavachalcone showed an obvious increase

while the contents of bavachromanol and bakuchiol decreased in salt-processed Psoralea Fructus. This could be the material foundation for the alternation of pharmacological effects after processing. Conclusion: By Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (OPLS-DA)

the indicative marker

like bavachinin

bavachalcone and bavachin

could be used to identify the crude and processed Psoralea Fructus. The experimental results can not only be of great importance to the processing principle of salt-processed Psoralea Fructus

but also provide crucial basis for clarifying effective material basic of salt-processed Psoralea Fructus.