JI Bao-yu, PEI Li-xin, CHEN Sui-qing, et al. RAPD Analysis of Germplasm Resource in [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 56-59.
JI Bao-yu, PEI Li-xin, CHEN Sui-qing, et al. RAPD Analysis of Germplasm Resource in [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 56-59. DOI: 10.13422/j.cnki.syfjx.2014160056.
Objective: This study aimed to establish the RAPD molecular marker system and to probe into the genetic background of Pueraria lobata. Method: RAPD analyses of selected plants from eleven various habitats in China were carried out using arbitrary primers
in combination with the chemical investigation of puerarin. Result: The percentage of puerarin ranged from 3.26% to 7.10%. A total of 45 DNA fragments were obtained using 8 primers
37 of which were polymorphic
accounting for 82.22%
which can tell that the samples had a high level of genetic diversity. Conclusion: P. lobata has significant genetic differentiation that further support the prospects for germplasm selection.