蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的测定

许科科; 李少华; 左长英; 陈朝勇; 张瑞桐; 兰明; 林玉; 张天娥; 闫智勇

doi:10.13422/j.cnki.syfjx.2014160064

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蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的测定

化学与分析 | 更新时间：2024-01-04

- 蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的测定
- Content Determination of Chlorovaltrate and Valjatrate B in Total Iridoids of Valerianae Jatamansi Rhizoma et Radix
- 中国实验方剂学杂志 2014年20卷第16期页码：64-66
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家“重大新药创制”科技重大专项（2009ZX09103-370）;教育部中央高校基本科研业务费专项资金（WJTU09CX063）
- DOI：10.13422/j.cnki.syfjx.2014160064
  中图分类号： R284.1
- 纸质出版日期：2014
- 稿件说明：
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许科科, 李少华, 左长英, 等. 蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的测定[J]. 中国实验方剂学杂志, 2014,20(16):64-66.

XU Ke-ke, LI Shao-hua, ZUO Chang-ying, et al. Content Determination of Chlorovaltrate and Valjatrate B in Total Iridoids of Valerianae Jatamansi Rhizoma et Radix[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 64-66.
许科科, 李少华, 左长英, 等. 蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的测定[J]. 中国实验方剂学杂志, 2014,20(16):64-66. DOI： 10.13422/j.cnki.syfjx.2014160064.

XU Ke-ke, LI Shao-hua, ZUO Chang-ying, et al. Content Determination of Chlorovaltrate and Valjatrate B in Total Iridoids of Valerianae Jatamansi Rhizoma et Radix[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 64-66. DOI： 10.13422/j.cnki.syfjx.2014160064.

摘要

目的：运用高效液相色谱法测定蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的含量。方法：采用高效液相色谱法，waters C18色谱柱（4.6 mm×250 mm，5 μm）；chlorovaltrate和蜘蛛香素B的流动相分别为乙腈-水（60：40），（50：50）；检测波长分别为254，203 nm；流速均为1.0 mL·min-1；柱温均为30℃。结果：蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B进样量分别在15.5~155，20~200 pg与各自峰面积积分值呈良好线性关系（r分别为0.998 6，0.998 7）；测得chlorovaltrate和蜘蛛香素B含量分别为8.91，7.00 mg·g-1。结论：确定了蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的含量，为蜘蛛香总环烯醚萜的质量控制提供了一定依据。

Abstract

Objective: The purpose of this study was to determine the content of chlorovaltrate and valjatrate B in total Iridoids of Valerianae Jatamansi Rhizoma et Radix. Method: HPLC method was adopted. The chromatographic column was waters C18 column (4.6 mm×250 mm

5 μm); the mobile phase for chlorovaltrate and valjatrate B was acetonitrile water (60:40)

(50:40); the detection wavelength for chlorovaltrate and valjatrate B were 254

203 nm; the flow rate was 1.0 mL· min-1; the column temperature was 30℃. Result: The fine linear correlation displayed when the injection volumn were 15.5-155 pg (r=0.998 6)

20-200 pg (r=0.998 7) for chlorovaltrate and valjatrate B in total iridoids of Valerianae Jatamansi Rhizoma et Radix

respectively. The contents of chlorovaltrate and valjatrate B were 8.91 mg· g-1 and 7.00 mg· g-1

respectively. Conclusion: The contents of chlorovaltrate and valjatrate B in total Iridoid of Valerianae Jatamansi Rhizoma et Radix were determined to provide evidence for quality control of total iridoids of Valerianae Jatamansi Rhizoma et Radix.

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