Objective: This study was aimed to investigate the chemical constituent of Kadsura longipeduculata collected from Guizhou province

and to explore the active ingredients in this herb. Method: Methods including silica gel column chromatography

macroporous resin column chromatography

molecular sieve chromatography

recrystallization and HPLC preparation were used. And the constituents were separated and purified from the petroleum ether and ethyl acetate fractions of ethanol extract of K. longipeduculata. According to their physical and chemical properties

coupled with the data of 1H-NMR

13C-NMR and EIS-MS

the structures were elucidated. Result: Eight compounds were isolated and identified as tubiferolide methyl ester(1)

schizandronic acid(2)

sorghumol(3)

schizandriside(4)

lancilactone C(5)

kadsurarin(6)

β-sitosterol(7)

(+)-anwulignan(8). Conclusion: Compounds 2-5 are isolated from the plant for the first time. 1

2

3

5 are triterpene compounds

and 4

6

8 are lignans compounds.