HPLC测定新疆紫草中紫草素的含量及提取方法的优化

崔晓秋; 刘云刚

doi:10.13422/j.cnki.syfjx.2014160094

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HPLC测定新疆紫草中紫草素的含量及提取方法的优化

化学与分析 | 更新时间：2024-01-04

- HPLC测定新疆紫草中紫草素的含量及提取方法的优化
- Ultrasonic-assisted Extraction and Content Determination of Shikonin from Arnebiae Radix by HPLC
- 中国实验方剂学杂志 2014年20卷第16期页码：94-97
- 作者机构：
- 作者简介：
- 基金信息：
  
  济宁医学院青年基金项目（JYQ2011KM027）
- DOI：10.13422/j.cnki.syfjx.2014160094
  中图分类号： O657.72;R284.1
- 纸质出版日期：2014
- 稿件说明：
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崔晓秋, 刘云刚. HPLC测定新疆紫草中紫草素的含量及提取方法的优化[J]. 中国实验方剂学杂志, 2014,20(16):94-97.

CUI Xiao-qiu, LIU Yun-gang. Ultrasonic-assisted Extraction and Content Determination of Shikonin from Arnebiae Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 94-97.
崔晓秋, 刘云刚. HPLC测定新疆紫草中紫草素的含量及提取方法的优化[J]. 中国实验方剂学杂志, 2014,20(16):94-97. DOI： 10.13422/j.cnki.syfjx.2014160094.

CUI Xiao-qiu, LIU Yun-gang. Ultrasonic-assisted Extraction and Content Determination of Shikonin from Arnebiae Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 94-97. DOI： 10.13422/j.cnki.syfjx.2014160094.

摘要

目的：采用超声方法提取新疆紫草中紫草素，并以左旋紫草素为指标，建立HPLC测定紫草素的含量。方法：利用超声波协同甲醇提取40 min；色谱条件为Shim-pack CLC-ODS-18色谱柱（4.6 mm×250 mm，10 μm），检测波长516 nm，流动相甲醇-水（85：15），流速1.0 mL·min-1，柱温室温。结果：左旋紫草素进样量在0.127~1.143 μg具有良好的线性关系，回归方程为 Y= 338 763X-198 347（r=0.999 8），平均回收率99.3%，RSD 1.18%，左旋紫草素超声法提取率是回流提取法的1.29倍。结论：经方法学验证，该实验所采用的实验方法重复性好、稳定、准确、可靠，可用于中药紫草药材中紫草素的含量测定。

Abstract

Objective: To develop a method for ultrasonic-assisted extraction and high performance liquid chromatography (HPLC) determination of shikonin in the roots of Arnebiae Radix. Method: Shikonin was extracted by ultrasonic-assisted-methyl alcohol method. The HPLC determination condition consisted of Shim-pack CLC-ODS-18 (4.6 mm×250 mm

10 μm) and shikonin was detected at 516 nm wavelength by using methyl alcohol-water(85∶ 15)as the mobile phase

column temperature at room temperature and flow rate 1.0 mL·min-1. Result: Shikonin had a good linearity relation in the range 0.127-1.143 μg (r= 0.999 8)

regressive equation were Y=338 763X-198 347. The average recovery was 99.3%

RSD 1.18% (n=6). In contrast with refluxing process

the extraction efficiency of ultrasonic extraction was 1.29 times. Conclusion: The methods of ultrasonic extraction and HPLC determination were proved to be accurate

reliable and repeatable. It can be used to determine natural shikonin content of Arnebiae Radix easily.

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