Objective: To establish a method for simultaneous determination of gardenin

geniposide

albiflorin

paeoniflorin

liquiritigenin

paeonol in Danzhi Xiaoyaosan by HPLC. Method: The Cosmosil 5 C18 column(4.6 mm×250 mm

5 μm)was used with a mobile phase of acetonitrile(A)- 0.5% phosphoric acid solution (B) gradient elution

the flow rate was 1 mL· min-1

and the detection wave-lengths were set at 230 nm. Result: The linear ranges were 0.009 46-0.946 mg· L-1 for gardenin

0.009 58-0.958 mg· L-1 for geniposide

0.008 68-0.868 mg· L-1 for albiflorin

0.004 88-0.488 mg· L-1 for paeoniflorin

25.0-500 mg· L-1 for liquiritigenin

25.0-500 mg· L-1 for paeonol respectively. The average recoveries of three constituents were 96.1%

104.3%

100.5%

94.7%

97.4%

95.4% with the RSD of 1.32%

2.55%

0.67%

1.29%

1.36%

2.38%. The contents of six compounds

gardenin

geniposide

albiflorin

paeoniflorin

liquiritigenin

paeonol in Danzhi Xiaoyaosan were 1.203

0.960

0.720

0.157

0.104

0.127 mg· g-1. Conclusion: Determination of Danzhi Xiaoyaosan could effectively control the product quality of Danzhi Xiaoyaosan.