五倍子有效部位抗病原微生物活性

代敏; 彭成; 叶强

doi:10.13422/j.cnki.syfjx.2014160147

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五倍子有效部位抗病原微生物活性

药理 | 更新时间：2024-01-04

- 五倍子有效部位抗病原微生物活性
- Antimicrobial Activity of Active-fractions from Ethanol Extract of Galla Chinensis
- 中国实验方剂学杂志 2014年20卷第16期页码：147-151
- 作者机构：
- 作者简介：
- 基金信息：
  
  四川省科技厅重点科技自筹项目（2011JYZ004）;四川省教育厅项目（11ZB206）;四川省卫生厅项目（130307）;成都医学院校基金（CYZ11-002）
- DOI：10.13422/j.cnki.syfjx.2014160147
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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代敏, 彭成, 叶强. 五倍子有效部位抗病原微生物活性[J]. 中国实验方剂学杂志, 2014,20(16):147-151.

DAI Min, PENG Cheng, YE Qiang. Antimicrobial Activity of Active-fractions from Ethanol Extract of Galla Chinensis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 147-151.
代敏, 彭成, 叶强. 五倍子有效部位抗病原微生物活性[J]. 中国实验方剂学杂志, 2014,20(16):147-151. DOI： 10.13422/j.cnki.syfjx.2014160147.

DAI Min, PENG Cheng, YE Qiang. Antimicrobial Activity of Active-fractions from Ethanol Extract of Galla Chinensis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 147-151. DOI： 10.13422/j.cnki.syfjx.2014160147.

摘要

目的：探讨五倍子抗细菌和抗白假丝酵母真菌的有效活性部位。方法：采用琼脂平板二倍稀释法测定了五倍子醇提物的石油醚、三氯甲烷、乙酸乙酯、正丁醇、乙醇和水6个有效部位对金黄色葡萄球菌、链球菌、大肠埃希菌等5个种属细菌和白假丝酵母真菌的最低抑菌浓度（MIC）。用单因素方差分析法、独立样本t检验比较分析不同活性部位对受试菌株抑菌活性的强弱。结果：五倍子石油醚、三氯甲烷和乙醇有效部位均无抗细菌和真菌活性，水部位仅对金黄色葡萄球菌有一定抗菌活性，MIC为733.69 g·L-1，正丁醇部位仅对5种细菌有较强的抗菌活性，MIC为2.06~32.96 g·L-1，而乙酸乙酯部位对受试细菌和白假丝酵母真菌均有强的抗菌活性，MIC分别为2.88~23.07 g·L-1和0.36~27.28 g·L-1。统计分析表明，乙酸乙酯和正丁醇部位对革兰阳性菌的抑菌活性强于对革兰阴性菌的抑菌活性（P<0.01，P<0.05），两者对同种属细菌的抑菌活性强弱相当，对耐药株和敏感株具有相似的抗菌活性。虽五倍子乙酸乙酯部位对白假丝酵母真菌临床分离株的MIC（95.15±96.34）mg·L-1明显高于克霉唑（8.00±8.92）mg·L-1（P<0.01），但克霉唑对临床分离株的耐药率为14.29% （2/14），而乙酸乙酯部位对所有临床分离株均有相似的抗菌活性（MIC为0.36~0.72 g·L-1）。结论：五倍子抗细菌和真菌的活性部位为乙酸乙酯部位。

Abstract

Objective: To investigate the antimicrobial fractions of Galla Chinensis. Method: The minimum inhibitory concentrations (MICs) of portions of petroleum ether

chloroform

ethyl acetate

n-butanol

ethanol and water of Galla Chinensis against five bacterias and Candida albicans were determined by agar dilution method. The data were analyzed using statistical software SPSS 17.0

and multifactor analysis of variance (ANOVA) and independent samples t test were used to compare the differences between 2 active fractions of ethyl acetate

n-butanol against same bacterial species

and between Clotrimazole and ethyl acetate fraction of Galla Chinensis against C. albicans. Result: The 3 portions of petroleum ether

chloroform and ethanol of Galla Chinensis had no effect on bacteria and C. albicans

and water fraction only had weak activity against S. aureus

with MICs 733.69 g·L-1

n-butanol fraction only had antibacterial activity

with MICs ranging from 2.06 g· L-1 to 32.96 g· L-1. However

ethyl acetate fraction of Galla Chinensis had strong antibacterial and antifungal activities

with MICs ranging from 2.88-23.07 g· L-1 and 0.36-27.28 g· L-1

respectively. The ethyl acetate fraction and n-butanol fraction of Galla Chinensis had higher activity against Gram-positive isolates than Gram-negative isolates (P<0.01

P<0.05)

and the two active-fractions had similar antibacterial activity against sensitive and resistant bacteria. The ethyl acetate fraction of Galla Chinensis had strong effect on C. albicans from sensitive and resistant clinical isolates

with MICs ranging from 0.36 g· L-1 to 0.72 g· L-1

but the resistance rate of Clotrimazole was 14.29% (2/14). Conclusion: Antibacterial and antifungal active fraction of G.chinensis was ethyl acetate fraction

which had broad-spectrum antimicrobial activity so to make it a potential candidate as antimicrobial agent to treat infectious diseases.

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