川芎嗪微乳凝胶预防大鼠实验性腹腔粘连抗炎及抗氧化作用

张慧; 宋宇; 张婷; 曾莉

doi:10.13422/j.cnki.syfjx.2014160159

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川芎嗪微乳凝胶预防大鼠实验性腹腔粘连抗炎及抗氧化作用

药理 | 更新时间：2024-01-04

- 川芎嗪微乳凝胶预防大鼠实验性腹腔粘连抗炎及抗氧化作用
- Anti-inflamamatory and Anti-peroxidation Action of Ligustrazine Microemulsion Gel in Ankylenteron Rats
- 中国实验方剂学杂志 2014年20卷第16期页码：159-164
- 作者机构：
- 作者简介：
- 基金信息：
  
  江苏省2012年度普通高校研究生科研创新计划项（CXZZ12_0620）;南京中医药大学基础研究与重点培育项目（09XPY05）
- DOI：10.13422/j.cnki.syfjx.2014160159
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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张慧, 宋宇, 张婷, 等. 川芎嗪微乳凝胶预防大鼠实验性腹腔粘连抗炎及抗氧化作用[J]. 中国实验方剂学杂志, 2014,20(16):159-164.

ZHANG Hui, SONG Yu, ZHANG Ting, et al. Anti-inflamamatory and Anti-peroxidation Action of Ligustrazine Microemulsion Gel in Ankylenteron Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 159-164.
张慧, 宋宇, 张婷, 等. 川芎嗪微乳凝胶预防大鼠实验性腹腔粘连抗炎及抗氧化作用[J]. 中国实验方剂学杂志, 2014,20(16):159-164. DOI： 10.13422/j.cnki.syfjx.2014160159.

ZHANG Hui, SONG Yu, ZHANG Ting, et al. Anti-inflamamatory and Anti-peroxidation Action of Ligustrazine Microemulsion Gel in Ankylenteron Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 159-164. DOI： 10.13422/j.cnki.syfjx.2014160159.

摘要

目的：观察川芎嗪微乳凝胶对肠粘连的防治效果，通过检测大鼠腹腔液细胞因子及粘连组织抗氧化酶进一步探讨其对肠粘连防治作用的机理。方法：取SD大鼠70只，随机分为7组：正常组、模型组、川芎嗪对照组、空白微乳凝胶组，川芎嗪微乳凝胶高、中、低剂量组，每组10只。除正常对照组外，其余各组大鼠均制备肠粘连模型。正常对照组和模型组ip生理盐水，川芎嗪对照组ip川芎嗪注射液（15 mg·kg-1·d-1），连续给药10 d。川芎嗪微乳凝胶低、中、高剂量组（7.5，15，30 mg·kg-1）在造模后，腹腔内以注射器均匀涂抹于创面，并按10 d 量放大，一次性给药。各组大鼠于术后第11天处死，ELISA法测定腹腔液白介素-6（IL-6）、白介素-18（IL-18）和肿瘤坏死因子α（TNF-α）含量，并取粘连组织检测超氧化物歧化酶（SOD）、一氧化氮合酶（NOS）、还原型谷胱甘肽（GSH）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）活力及丙二醛（MDA）、一氧化氮（NO）含量，同时记录大鼠肠粘连级别。取粘连肠组织观察病理变化。结果：与正常组相比，模型组腹腔液IL-6，IL-18和TNF-α含量增高（P<0.01），粘连组织中MDA，NO，NOS活性增强（P<0.01），CAT，GPx，GSH，SOD的活性下降（P<0.01）。与模型组相比，川芎嗪微乳凝胶低、中、高剂量组的肠粘连程度明显减轻（P<0.05，P<0.01），病理显示肠粘连逐渐松解，腹腔液IL-6，IL-18和TNF-α含量明显降低（P<0.05，P<0.01），粘连组织中MDA，NO含量及NOS活性明显下降（P<0.05，P<0.01），CAT，GPx，GSH，SOD的活性增强（P<0.05，P<0.01）。结论：川芎嗪微乳凝胶可明显减轻肠粘连的程度，对肠粘连具有防治作用。其作用可能是通过下调IL-18的表达，进而使相关基因的表达下调，导致下游炎性因子TNF-α，IL-6等表达减少，同时与抗肠组织过氧化损伤有关。

Abstract

Objective: To investigate the effect of ligustrazine microemulsion gel on the expression of ctokine in peritoneal fluid and antioxidase in the ahension tissue in rats with peritoneal adhesion. Method: Seventy SD rats were randomly divided into 7 groups:normal control group

model group

blank microemulsion gel groups (5 mL· kg-1)

ligustrazine group(15 mg· kg-1) and low-

medium-and high-dose ligustrazine microemulsion gel groups(in the dose of 7.5

30 mg·kg-1 respectively).Except the normal control group

the rats in other groups were induced ankylenteron. Intraperitoneal injection of saline was administered to the rats in the normal control group and the model group

and ligustrazine groups were given ligustrazine injection to abdominal cavity.The treatment lasted 10 days.In the low-

medium-and high-dose ligustrazine microemulsion gel groups

the microemulsion gel were scribbled evenly on the wound by syringe in the abdominal cavity after modeling.On the 11th day after surgery

the peritoneal fluid and the adhesion tissue of each group were collected.The levels of interleukin-18(IL-18)

interleukin-6(IL-6) and tumor necrosis factor-a(TNF-a) in peritoneal fluid were determined by enzyme linked immunosorbent assay and the contents of superoxide dismutase (SOD)

nitric oxide synthase (NOS)

glutathione(GSH)

Malondialdehyde(MDA)

nitric oxide(NO)

catalase(CAT)

glutathione peroxidase(GPx) in the adhesion tissue were measured by kits.Grades of intestinal adhesion were ranked by macroscopic observation.The intestine tissue were adopted to observe the change of pathomorphology. Result: Comared with the contol group

ligustrazine microemulsion gel could significantly relieve the experimental intestinal adhesion and decreased the levels of IL-18

IL-6 and TNF-α in peritoneal fluid obviously(P<0.05

P<0.01).The activity of MDA

NOS in the adhesion tissue were reduced significantly(P<0.05

P<0.01)

but the activity of CAT

GPx

GSH

SOD were increased markedly(P<0.05

P<0.01)in the low-

medium-and high-dose ligustrazine microemulsion gel groups. Pathomorphology investigation showed that small intestine injury was restored in low-

medium-and high-dose ligustrazine groups. Conclusion: Ligustrazine microemulsion gel can effectively prevent the formation of postoperative ankylenteron by inhibiting the expression of inflammatory cytokines and be correlated with anti-intestine tissue peroxidation.

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