枸杞总多糖对2型糖尿病大鼠细胞因子的影响

尹长江; 杨坤宝; 张学军; 史华宁; 张海峰; 宋鸿儒

doi:10.13422/j.cnki.syfjx.2014160169

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枸杞总多糖对2型糖尿病大鼠细胞因子的影响

药理 | 更新时间：2024-01-04

- 枸杞总多糖对2型糖尿病大鼠细胞因子的影响
- The Effect of Lycium Barbarum Polysaccharides on Cytokines in Type 2 Diabetic Rats
- 中国实验方剂学杂志 2014年20卷第16期页码：169-172
- 作者机构：
- 作者简介：
- 基金信息：
  
  河北省教育厅科技-青年基金项目（QN20131039）
- DOI：10.13422/j.cnki.syfjx.2014160169
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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尹长江, 杨坤宝, 张学军, 等. 枸杞总多糖对2型糖尿病大鼠细胞因子的影响[J]. 中国实验方剂学杂志, 2014,20(16):169-172.

YIN Chang-jiang, YANG Kun-bao, ZHANG Xue-jun, et al. The Effect of Lycium Barbarum Polysaccharides on Cytokines in Type 2 Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 169-172.
尹长江, 杨坤宝, 张学军, 等. 枸杞总多糖对2型糖尿病大鼠细胞因子的影响[J]. 中国实验方剂学杂志, 2014,20(16):169-172. DOI： 10.13422/j.cnki.syfjx.2014160169.

YIN Chang-jiang, YANG Kun-bao, ZHANG Xue-jun, et al. The Effect of Lycium Barbarum Polysaccharides on Cytokines in Type 2 Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 169-172. DOI： 10.13422/j.cnki.syfjx.2014160169.

摘要

目的：探讨枸杞总多糖（lycium barbarum polysaccharide，LBP）对2型糖尿病（NIDDM）大鼠细胞因子的调节作用。方法：采用高脂高糖饮食联合小剂量注射链尿佐菌素（streptozotocin，STZ）的方法制备NIDDM大鼠模型，随机选取40只均分为5组：模型组，LBP低（100 mg·kg-1）、中（200 mg·kg-1）、高（400 mg·kg-1）剂量干预组，格列苯脲干预组（阳性药物组）；同时设立基础饲料喂养的正常对照组。Western blot法检测LBP对NIDDM大鼠脾脏组织中白细胞介素-1β （IL-1β）、白细胞介素-6 （IL-6）、肿瘤坏死因子-α（TNF-α）表达的影响。结果：与正常对照组比较，NIDDM对照组大鼠脾脏组织中IL-1β，IL-6，TNF-α蛋白的表达均显著增高；以不同剂量的LBP干预治疗糖尿病大鼠4周后，各治疗组大鼠脾脏组织中IL-1β，IL-6，TNF-α的表达均显著降低。结论： LBP对NIDDM大鼠的免疫功能的调节作用可能与其抑制IL-1β，IL-6，TNF-α蛋白的表达有关。

Abstract

Objective: This study aimed to investigate the regulation roles of lycium barbarum polysaccharides (LBP) on cytokines in type 2 diabetic (NIDDM) rats. Method: High-fat and high-sugar diet in combination with low-dose injection of streptozotocin(STZ) was used to prepare type 2 diabetic (NIDDM) rat model. Forty diabetic immune model rats were randomly selected to divide into five groups:model group

low dose of LBP(100 mg· kg-1) intervention group

medium dose of LBP (200 mg· kg-1) intervention group

high dose of LBP (400 mg· kg-1) intervention group

glibenclamide intervention group (positive drug group). Rats in similar weight fed with basal diet were used as normal control group. Western bolt assay was used to detect the effect of LBP on the expression of interleukin-1β (IL-1β)

interleukin-6 (IL-6)

tumor necrosis factor-α(TNF-α) in type 2 diabetic rats' spleen tissue. Result: Compared with the normal control group

the expression of IL-1β

IL-6

TNF-α in the spleen tissue of NIDDM rats was significantly increased. After 4 weeks' intervention of different doses of LBP

the expression of IL-1β

IL-6

TNF-α in diabetic rats' spleen tissue in each treatment group significantly decreased. Conclusion: LBP may regulate the immune function of type 2 diabetic rats by inhibiting the expression of IL-1β

IL-6 and TNF-α.

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