Objective: To optimize extraction technology of Hirudo Zhusan. Method: The content of antithrombin activity substance was determined by anti-thrombin titration.Taking grinding degree as index to screen smash equipment

then effect of water content on powder of Hirudo Zhusan was investigated;with extract yield and transfer rate of antithrombin activity substance as indexes

single factor tests were adopted to optimize extraction process by taking soaking time

the amount of water

decoction time and so on as factors

then compared with traditional pieces of Hirudo. Result: Optimum extraction parameters was as follows:soaked 10 min with 20 times the amount of water

decocted thrice at 100 ℃ for 20 min each time;transfer rate of antithrombin activity substance could reach as highly as 93.31%

which was significantly higher than traditional pieces of 52.44%. Conclusion: This optimized process was stable and feasible

Hirudo Zhusan had advantages of high efficiency

short time

low energy consumption

environmental protection and so on

it could provide an insight for industrial production and new drug development of Hirudo Zhusan.