大孔吸附树脂纯化核桃内种皮总多酚的工艺优选

张春梅; 陈朝银; 赵声兰; 谢定岐; 樊启猛

doi:10.13422/j.cnki.syfjx.2014170027

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大孔吸附树脂纯化核桃内种皮总多酚的工艺优选

工艺与制剂 | 更新时间：2024-01-04

- 大孔吸附树脂纯化核桃内种皮总多酚的工艺优选
- Optimization of Purification Technology for Total Polyphenols from Juglandis Semen Kernel Pellicles by Macroporous Resin
- 中国实验方剂学杂志 2014年20卷第17期页码：27-30
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技部科技支撑计划项目（2011BAD46B03，2011BAD46B02）;国家中医药管理局傣药学学科建设科研项目（30970101808）
- DOI：10.13422/j.cnki.syfjx.2014170027
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
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张春梅, 陈朝银, 赵声兰, 等. 大孔吸附树脂纯化核桃内种皮总多酚的工艺优选[J]. 中国实验方剂学杂志, 2014,20(17):27-30.

ZHANG Chun-mei, CHEN Chao-yin, ZHAO Sheng-lan, et al. Optimization of Purification Technology for Total Polyphenols from Juglandis Semen Kernel Pellicles by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 27-30.
张春梅, 陈朝银, 赵声兰, 等. 大孔吸附树脂纯化核桃内种皮总多酚的工艺优选[J]. 中国实验方剂学杂志, 2014,20(17):27-30. DOI： 10.13422/j.cnki.syfjx.2014170027.

ZHANG Chun-mei, CHEN Chao-yin, ZHAO Sheng-lan, et al. Optimization of Purification Technology for Total Polyphenols from Juglandis Semen Kernel Pellicles by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 27-30. DOI： 10.13422/j.cnki.syfjx.2014170027.

摘要

目的：优选大孔吸附树脂纯化核桃内种皮总多酚的工艺条件。方法：以核桃内种皮为原料，采用乙醇回流法提取总多酚，通过静态吸附-洗脱试验筛选大孔吸附树脂型号，通过单因素试验考察上样液质量浓度、上样量、吸附流速、洗脱液浓度和用量等对核桃内种皮总多酚大孔树脂纯化工艺的影响。结果：选择X-5型大孔树，其最佳纯化工艺条件为上柱液pH 5.0，质量浓度3 g·L-1，吸附流速3 BV·h-1，上样量9.5 BV，加70%乙醇2 BV洗脱，收集洗脱液；总多酚纯度由粗提物的64.52%提高至79.64%，转移率89.83%。结论：优选的纯化工艺稳定可行，X-5型大孔树脂适用于核桃内种皮总多酚的初步纯化。

Abstract

Objective: To optimize purification technology of total polyphenols from Juglandis Semen kernel pellicles by macroporous resin. Method: With Juglandis Semen kernel pellicles as raw material

total polyphenols was extracted by ethanol refluxing method

static adsorption-elution test for total polyphenols were carried out to select proper macroporous resin

single factor tests were adopted to optimize purification process by taking the concentration of sample solution

adsorption flow rate

eluent dosage and so on as factors. Result: X-5 macroporous resin showed the best separation efficiency by comparing with other resins

its optimum purification technology was as follows:the concentration of sample solution 3.0 g·L-1 with pH of 5.0

adsorption rate of 3 BV·h-1

sample volume of 9.5 BV

eluted with 2 BV of 70% ethanol

collected eluent;Purity of total polyphenol was enhanced from 64.52% to 79.64% with transfer rate of 89.83% in extract. Conclusion: This optimized process was stable and feasible

X-5 resin was suitable for initial purification of total polyphenols from Juglandis Semen kernel pellicles.

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