正交试验法优选痔疮熏洗液的水提醇沉工艺

陈朝; 伍小燕; 黄一挚; 龚敏阳; 莫小林; 黄权芳

doi:10.13422/j.cnki.syfjx.2014170033

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正交试验法优选痔疮熏洗液的水提醇沉工艺

工艺与制剂 | 更新时间：2024-01-04

- 正交试验法优选痔疮熏洗液的水提醇沉工艺
- Optimization of Water Extraction and Alcohol Precipitation Technology for Hemorrhoid Fumigants by Orthogonal Test
- 中国实验方剂学杂志 2014年20卷第17期页码：33-36
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西壮族自治区中医药管理局项目（GZZJ13-05）
- DOI：10.13422/j.cnki.syfjx.2014170033
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
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陈朝, 伍小燕, 黄一挚, 等. 正交试验法优选痔疮熏洗液的水提醇沉工艺[J]. 中国实验方剂学杂志, 2014,20(17):33-36.

CHEN Chao, WU Xiao-yan, HUANG Yi-zhi, et al. Optimization of Water Extraction and Alcohol Precipitation Technology for Hemorrhoid Fumigants by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 33-36.
陈朝, 伍小燕, 黄一挚, 等. 正交试验法优选痔疮熏洗液的水提醇沉工艺[J]. 中国实验方剂学杂志, 2014,20(17):33-36. DOI： 10.13422/j.cnki.syfjx.2014170033.

CHEN Chao, WU Xiao-yan, HUANG Yi-zhi, et al. Optimization of Water Extraction and Alcohol Precipitation Technology for Hemorrhoid Fumigants by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 33-36. DOI： 10.13422/j.cnki.syfjx.2014170033.

摘要

目的：优选痔疮熏洗液的水提醇沉工艺。方法：以总生物碱含量和干膏率的综合评分为指标，通过正交试验考察加水量、提取次数、提取时间对水提工艺的影响；以总生物碱含量为指标，运用正交试验考察醇沉浓度、放置时间、浓缩液相对密度对醇沉工艺的影响。采用UV测定总生物碱含量，检测波长416 nm。结果：最佳水提醇沉工艺为加10倍量水煎煮2次，每次1.5 h，提取液浓缩至相对密度1.06 g·mL-1（25 ℃），醇沉至乙醇体积分数60%，放置时间12 h；总生物碱质量浓度2.040 g·L-1。结论：优选的提取、醇沉工艺稳定可行，为痔疮熏洗液的规范化生产提供参考。

Abstract

Objective: To optimize water extraction and alcohol precipitation technology of hemorrhoid fumigants. Method: With composite score of the content of total alkaloids and dry extract yield as index

effects of the amount of water

extraction times and time on extraction technology was investigated by orthogonal test;with the content of total alkaloids as index

orthogonal test was adopted to investigate effects of relative density of extract

concentration and time of alcohol precipitation on alcohol precipitation technology.UV was employed to determine the content of total alkaloids with detection wavelength at 416 nm. Result: The best water extraction and alcohol precipitation technology was as following:decocted twice with 10 times the amount of water for 1.5 h per time

relative density of extract of 1.06 g·mL-1 at 25 ℃

alcohol precipitation concentration 60%

storage time of 12 h;the concentration of total alkaloids was 2.040 g·L-1. Conclusion: These optimized technologies were stable and feasible to provide a reference for standardized production of hemorrhoid fumigants.

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