正交试验法优选泰痹颗粒的提取工艺

叶淑颖; 唐业建; 曾怡; 郭婉媚; 王岩

doi:10.13422/j.cnki.syfjx.2014170037

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正交试验法优选泰痹颗粒的提取工艺

工艺与制剂 | 更新时间：2024-01-04

- 正交试验法优选泰痹颗粒的提取工艺
- Optimization of Extraction Technology for Taibi Granules by Orthogonal Test
- 中国实验方剂学杂志 2014年20卷第17期页码：37-40
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西科技攻关项目（桂科攻-12118005-2-8）
- DOI：10.13422/j.cnki.syfjx.2014170037
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
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叶淑颖, 唐业建, 曾怡, 等. 正交试验法优选泰痹颗粒的提取工艺[J]. 中国实验方剂学杂志, 2014,20(17):37-40.

YE Shu-ying, TANG Ye-jian, ZENG Yi, et al. Optimization of Extraction Technology for Taibi Granules by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 37-40.
叶淑颖, 唐业建, 曾怡, 等. 正交试验法优选泰痹颗粒的提取工艺[J]. 中国实验方剂学杂志, 2014,20(17):37-40. DOI： 10.13422/j.cnki.syfjx.2014170037.

YE Shu-ying, TANG Ye-jian, ZENG Yi, et al. Optimization of Extraction Technology for Taibi Granules by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 37-40. DOI： 10.13422/j.cnki.syfjx.2014170037.

摘要

目的：优选泰痹颗粒的提取工艺。方法：以虎杖苷、大黄素含量及干膏得率的综合评分为指标，采用L9（34）正交试验法考察加水量、提取次数及提取时间对泰痹颗粒水提工艺的影响。采用RP-HPLC测定虎杖苷、大黄素含量，流动相分别为乙腈-水（21：79）和甲醇-0.1%磷酸溶液（77：23），检测波长依次为306，254 nm。结果：最佳提取工艺为加10倍量水提取3次，每次1 h；虎杖苷、大黄素质量分数及干膏得率的平均值分别为0.076%，0.510%，25.720%。结论：优选的提取工艺稳定可行，适用于该制剂的工业生产。

Abstract

Objective: To optimize water extracting technology of Taibi granules. Method: With dry extract yield

contents of polydatin and emodin as comprehensive evaluation indexes

L9(34) orthogonal test was adopted to optimize extraction process by taking the amount of water

extraction times and time as factors.Contents of polydatin and emodin were determined by RP-HPLC

mobile phases were acetonitrile-water(21:79) and methanol-0.1% phosphoric acid solution(77:23)

detection wavelengths were 306

254 nm

respectively. Result: Optimum extraction process was as follows:refluxing extracted 3 times with 10-fold water

1 h for each time;average values of dry extract yield

mass fractions of polydatin and emodin were 25.720%

0.076% and 0.510%

respectively. Conclusion: Optimized extraction technology was stable

feasible and suitable for industrial production of Taibi granules.

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