Objective: To establish a HPLC method for determining the content of solasonine in different extracts of Solanum torvum fruits. Method: Solasonine from S. torvum fruits was extracted by system solvent extraction method

contents of solasonine in different extracting parts were compared.The content of solasonine was determined by HPLC with mobile phase of acetonitrile-0.2% phosphoric acid aqueous solution(15:85)

flow rate of 0.5 mL·min-1

detection wavelength at 250 nm and column temperature at 30 ℃. Result: Solanine showed a good linear relationship within 2-80 μg

average recovery was 100.93% with RSD of 0.60%.Elution parts of water

10% ethanol

30% ethanol

50% ethanol

70% ethanol

95% ethanol and extracting parts in petroleum ether

ethyl acetate

n-butanol

water were 0.062 9

0.939 0

0.020 5

0.118 0

0.005 1

0.004 4

0.010 6

0.031 4

0.070 5

0.113 0 mg·g-1

respectively. Conclusion: This method was simple

accurate and specific

it could be considered as an effective method for determinng the content of solasonine in different extracts of S. torvum fruits

which had quite difference in different extracting parts.