Objective: This study was performed to evaluate the quality of Euodiae Fructus from different varieties and locations and to provide the foundation for quality control it in the market by UPLC-UV fingerprint and content determination of active compounds of limonin

evodiamine and rutaecarpine. Method: The separation was performed on an ACQUITY UPLC HSS T3 column (2.1 mm×100 mm

1.8 μm) with gradient elution using acetonitrile-water asmobile phase. The flow rate was 0.5 mL·min-1. UV detecting wavelength was set at 210 nm

and column temperature was set at 25 ℃. The holistic quality of 24 samples were evaluated by fingerprint combined with unsupervised principal component analysis (PCA). The three active compounds in 24 samples collected from different varieties and locations were determined. Result: An ultra-performance liquid chromatographic method was established for fingerprint analysis

and nine peaks in UPLC profile were found as the same. The result of fingerprint similarity evaluation showed that the quality of Euodiae Fructus collected from Guizhou was stable and consistent

however

that from Zhejiang was inconsistent slightly. The result of PCA showed that there is difference in the components of Euodiae Fructus from different varieties. The contents of limonin

evodiamine and rutaecarpine in Euodiae Frutus were influenced by locations. Conclusion: The fingerprint analysis combined with the content determination of active compounds was available for holistic quality evaluation of Euodiae Fructus

which is directive is directive significance to origin identification and quality control of Euodiae Fructus.