Objective: This study was conducted to establish a systematic method for the qualitative and quantitative analysis of the major contents from Polygonati Odorati Rhizoma. Method: TLC method was used to identify the authenticity of Polygonati Odorati Rhizoma by TLC

while HPLC-LISD method was applied to determinate its saponins POD I by HPLC-ELSD. The TCL conditions included three developing systems:A consisted of petroleum ether

chloroform

methanol and formic acid with the ratio of 1:9:0.65:0.05

B consisted of chloroform

acetone and formic acid with the ratio of 7:1.1:0.05 and C consisted of cyclohexane

ethyl acetate

n-butanol and formic acid with the ratio of 5:2:0.3:0.03.Chromogenic agents were composed of 1% potassium ferricyanide and 1% mixing ferric chloride ethanol solution(1:1). Chromatographic separation was carried out on an Agilent ZORBAX SB-C18chromatographic column (4.6 mm×250 mm

5 μm). The chromatographic conditions were as follows:flow rate of 1.0 mL·min-1;sample injection volume of 10 μL;mobile phase A (acetonitrile-methanol) and mobile phase B (water). For ELSD detection

the operating parameters were as follows:N2 low rate of 1.6 L·min-1;drift tube temperature of 42.6 ℃;gain value of 4. Result: TLC method could be omployed to distinguish Rhizoma Polygonati Odorati from its fake effectively. There was a good linear relationship for PODI in the range of 0.514 5-9.261 0 μg (r=0.999 7)

and the average recovery (n=6) was 103.09% with the RSD of 1.52%. Conclusion: The method was simple

accurate and reliable

and it might provide the basis for the more effective quality control of Polygonati Odorati Rhizoma.