Objective: The purpose of this study was to optimize the extraction technology of protein in Gekko Swinhoanis. Method: The proper extraction method was selected by comparing the effect of ultrasonic and reflux extraction on protein extraction rate of Gekko Swinhoanis. Then single factor analysis was done for four influencing factors

such as pH

ration of liquid to material

temperature and time. The optimized extraction technology was investigated using orthogonal test with the content of protein as the index for the first time. Result: The protein content by ultrasonic extraction was significantly higher than that by reflux extraction. The best extraction condition of protein in Gekko Swinhoanis was proposed

that's the powder of Gekko Swinhoanis was extracted for 80 minutes with 20 times amount of water (pH 12) by ultrasonic. Conclusion: The optimum extraction condition was stable

reliable and feasible

and could provide a experimental basis for formulating the quality standards and further study of bioactive substances of traditional Chinese medicine Gekko Swinhoanis.