HPLC同时测定养阴清肺颗粒中芍药苷、甘草苷和肉桂酸含量

张咪咪; 齐广才; 张越诚; 刘阿静; 刘珍叶

doi:10.13422/j.cnki.syfjx.2014170088

您当前的位置：

首页 >

文章列表页 >

HPLC同时测定养阴清肺颗粒中芍药苷、甘草苷和肉桂酸含量

化学与分析 | 更新时间：2024-01-04

- HPLC同时测定养阴清肺颗粒中芍药苷、甘草苷和肉桂酸含量
- Simultaneous Determination of Paeoniflorin, Liquiritin and Cinnamic Acid in Yangyin Qingfei Granules by RP-HPLC
- 中国实验方剂学杂志 2014年20卷第17期页码：88-90
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014170088
  中图分类号： O657.72;R286.0
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
张咪咪, 齐广才, 张越诚, 等. HPLC同时测定养阴清肺颗粒中芍药苷、甘草苷和肉桂酸含量[J]. 中国实验方剂学杂志, 2014,20(17):88-90.

ZHANG Mi-mi, QI Guang-cai, ZHANG Yue-cheng, et al. Simultaneous Determination of Paeoniflorin, Liquiritin and Cinnamic Acid in Yangyin Qingfei Granules by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 88-90.
张咪咪, 齐广才, 张越诚, 等. HPLC同时测定养阴清肺颗粒中芍药苷、甘草苷和肉桂酸含量[J]. 中国实验方剂学杂志, 2014,20(17):88-90. DOI： 10.13422/j.cnki.syfjx.2014170088.

ZHANG Mi-mi, QI Guang-cai, ZHANG Yue-cheng, et al. Simultaneous Determination of Paeoniflorin, Liquiritin and Cinnamic Acid in Yangyin Qingfei Granules by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 88-90. DOI： 10.13422/j.cnki.syfjx.2014170088.

摘要

目的：建立以高效液相色谱法同时测定养阴清肺颗粒中芍药苷、甘草苷和肉桂酸含量的方法。方法：采用双波长切换-高效液相色谱法对养阴清肺颗粒中芍药苷、甘草苷和肉桂酸进行定量分析。Agilent ZORBAX SB-C18 色谱柱（4.6 mm×250 mm，5 μm），流动相0.2%磷酸水-乙腈梯度洗脱，检测波长276，230 nm，柱温28 ℃，流速1.0 mL·min-1。结果：芍药苷、甘草苷和肉桂酸分别在0.236 8~4.736 mg·L-1（r=0.999 9），0.170 8~3.416 mg·L-1（r=0.999 9），0.035 2~0.704 mg·L-1（r=0.999 9）线性关系良好；平均回收率分别为99.74%，98.82%，98.40%。RSD分别为0.78%，0.97%，1.04%。结论：该方法简便、快速、准确，可用于养阴清肺颗粒的质量控制。

Abstract

Objective: The study was performed to establish a HPLC method for simultaneous determination of paeoniflorin

liquiritin and cinnamic acid in Yangyin Qingfei granules. Method: Paeoniflorin

liquiritin and cinnamic acid were determined simultaneously by HPLC on a ZORBAX SB-C18 column with the mobile phase composed of acetonitrile and 0.2% phosphoric acid in gradient elution. Detection wavelength were set at 276 nm and 230 nm.The column temperature was maintained at 28 ℃. The flow rate was fixed at 1.0 mL·min-1. Result: The calibration curves of paeoniflorin

liquiritin and cinnamic acid showed good linearity within 0.2368-4.736 mg·L-1 (r=0.999 9)

0.170 8-3.416 mg·L-1 (r=0.999 9)

0.035 2-0.70 4 mg·L-1 (r=0.999 9)

respectively. The average recoveries of those were 99.74%

98.82% and 98.40%

respectively. RSDs were 0.78%

0.97% and 1.04%

respectively. Conclusion: The developed HPLC method is fast

simple and accurate and can be used for quality control of Granules of Yangyin Qingfei.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

经典名方芍药甘草汤的物质基准量值传递分析

丹芪祛瘀止痛颗粒质量标准的研究

HPLC同时测定妇炎康片中芍药苷、丹酚酸B、小檗碱

中药炮制辅料甘草汁的质量标准研究

HPLC测定复方昆丹胶囊中芍药内酯苷、芍药苷、二苯乙烯苷、五没食子酰葡萄糖