Objective: This study was carried out to investigate the influence of different compatibility combinations of Simiao Yongan tang on the extraction efficiency of sweroside and loganin. Method: The extraction rates of sweroside and loganin were determined by HPLC

using a SunFireTM C18 analytical column (4.6 mm×150 mm

5 μm) in prescription dismantling of Simiao Yongan tang. The mobile phase was composed of aqueous phosphoric acid (0.1%) and acetonitrile using a gradient elution. Analytes were performed at 35 ℃ with a flow rate of 1.0 mL·min-1 and a UV detection at 250 nm. Result: The sweroside and loganin in eight groups from the original prescription and dismantlementwere detected and analyzed. The regression equations for sweroside and loganin were Y=1 179 101.85X-28 583.08(r=0.999 2) and Y=1 353 095.24X-4 113.75 (r=0.999 1)

respectively. The liner ranges were 0.191 2-1.338 4 μg for sweroside and 0.026 4-0.184 8 μg for loganin. The average recoveries of sweroside and loganin were 101.19% (RSD 2.83%) and 102.59% (RSD 2.30%)

respectively. Conclusion: Compared with single Lonicerae Japonicae Flos

the extraction rates of sweroside and loganin were improved in other compatibilities of Simiao Yongan tang. The whole prescription was superior to the other groups

which indicated that compatibility was preponderant and reasonable.