Objective: The Ginseng Radix et Rhizoma was biotransformed under the optimal condition to obtain the new product medicinal fungal substance of ginseng. This study was conducted to investigate the chemical constituents of the ethyl acetate fraction of medicinal fungal substance ginseng

to clarify the substance basis of efficacy. Method: Thin-layer chromatography

column chromatography on silica gel

Sephadex LH-20 gel chromatography and semi-preparative liquid chromatography were used for isolation and purification of the compounds

and their structures were determined by comparison of their chromatographic and spectral data with those of the authentic samples and those reported in the literatures. Result: Six compounds were identified as phthalate acid bis(2-ethylhexyl)ester (1)

dibutylphthalate (2)

stigmasta-5

22-dien-3-beta-ol (3)

a-sitosterol(4)

stigmast-22-ene-3

6-dione (5)

stigmasta-3

6-dione (6). Conclusion: All compounds were isolated from the medicinal fungal substance of Ginseng Radix et Rhizoma for the first time.