艾纳香不同部位提取物的抗氧化活性及其对酪氨酸酶的抑制作用

庞玉新; 袁蕾; 王中洋; 胡璇; 孙懂华; 杨全

doi:10.13422/j.cnki.syfjx.2014180004

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艾纳香不同部位提取物的抗氧化活性及其对酪氨酸酶的抑制作用

工艺与制剂 | 更新时间：2024-01-04

- 艾纳香不同部位提取物的抗氧化活性及其对酪氨酸酶的抑制作用
- Antioxidant Activities and Anti-tyrosinase of Extracts from Different Parts of
- 中国实验方剂学杂志 2014年20卷第18期页码：4-8
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81374065/81303171）;国家农业部引进国际先进农业科学技术计划（948计划）项目（2013S3）
- DOI：10.13422/j.cnki.syfjx.2014180004
  中图分类号： R284
- 纸质出版日期：2014
- 稿件说明：
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庞玉新, 袁蕾, 王中洋, 等. 艾纳香不同部位提取物的抗氧化活性及其对酪氨酸酶的抑制作用[J]. 中国实验方剂学杂志, 2014,20(18):4-8.

PANG Yu-xin, YUAN Lei, WANG Zhong-yang, et al. Antioxidant Activities and Anti-tyrosinase of Extracts from Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 4-8.
庞玉新, 袁蕾, 王中洋, 等. 艾纳香不同部位提取物的抗氧化活性及其对酪氨酸酶的抑制作用[J]. 中国实验方剂学杂志, 2014,20(18):4-8. DOI： 10.13422/j.cnki.syfjx.2014180004.

PANG Yu-xin, YUAN Lei, WANG Zhong-yang, et al. Antioxidant Activities and Anti-tyrosinase of Extracts from Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 4-8. DOI： 10.13422/j.cnki.syfjx.2014180004.

摘要

目的：考察艾纳香不同部位提取物的抗氧化活性及其对酪氨酸酶的抑制作用，筛选具有抗氧化活性及美白作用的有效部位。方法：采用水，50%乙醇及95%乙醇3种不同极性溶剂分别回流提取艾纳香的功能叶、嫩叶、嫩茎；运用DPPH，ABTS，FRAP法及酪氨酸酶抑制活性试验分别比较各部分提取物的相关活性。结果：艾纳香功能叶、嫩叶、嫩茎的95%乙醇提取物DPPH自由基清除率大于其他溶剂提取物，依次为95.38%，91.32%，92.49%；ABTS，FRAP法及酪氨酸酶抑制试验结果显示艾纳香功能叶和嫩叶的50%乙醇和95%乙醇提取物均具有较好的活性，当各提取物质量浓度为1 500 mg·L-1时，艾纳香功能叶的水，50%乙醇和95%乙醇提取物对ABTS自由基的清除率分别为26.54%，82.48%，67.46%，嫩叶的3种提取物分别为34.15%，95.39%，59.59%，嫩茎的3种提取物分别为52.05%，39.35%，38.77%。功能叶的水，50%乙醇及95%乙醇提取物对酪氨酸酶的抑制率分别为24.24%，32.79%，37.97%；嫩叶不同提取物的抑制率分别为27.12%，36.53%，34.19%，嫩茎的则依次为31.36%，26.22%，24.00%。结论：艾纳香提取物具有较好的抗氧化活性和酪氨酸酶抑制作用，其中功能叶和嫩叶为其主要活性部位。

Abstract

Objective: To investigate anti-tyrosinase and in vitro antioxidant activities of crude extracts from different parts of Blumea balsamifera

in order to screen active ingredients with whitening and antioxidant effects. Method: Functional leaves

tender leaves and immature stems of B. balsamifera were refluxed by aqueous

50% ethanol and 95% ethanol

respectively.Then crude extracts were measured tyrosinase inhibitory by enzymology method

and antioxidant activities were detected by DPPH

ABTS and FRAP methods. Result: DPPH free radical scavenging rate of 95% ethanol extract from functional leaves

tender leaves

immature stems of B. balsamifera was higher than other extracts

which was 95.38%

91.32% and 92.49%

respectively.FRAP

ABTS and anti-tyrosinase experiment's result showed that 50% ethanol and 95% ethanol extracts from functional leaves and immature stems possessed good activity.When concentration of each extracts were 1 500 mg·L-1

scavenging rates of aqueous

50% ethanol and 95% ethanol extracts of functional leaves for ABTS radical were 26.54%

82.48% and 67.46%

data of these three extracts of tender leaves were 34.15%

95.39% and 59.59%

while these data of immature stems were 52.05%

39.35% and 38.77%

respectively.Inhibition rates of aqueous

50% ethanol and 95% ethanol extracts of functional leaves on tyrosinase were 24.24%

32.79%

37.97%;these data of tender leaves were 27.12%

36.53% and 34.19%

while immature stems' were 31.36%

26.22% and 24.00%

respectively. Conclusion: Crude extracts from different parts of B. balsamifera have good antioxidant activities and inhibitory effect on tyrosinase

functional leaves and tender leaves are main active parts.

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