Objective: To optimize extraction process of total polysaccharides from fruiting body of Phellinus igniarius and preliminary analyze its monosaccharide composition. Method: The content of total polysaccharides was determined by phenol-sulfuric acid method.With yield of total polysaccharides as index

single factor tests and orthogonal design were adopted to optimize extraction process by taking liquid-solid ratio

extracting time and times as factors

monosaccharide composition of total polysaccharides from different origin and species of P. igniarius was determined by TLC. Result: Optimum extraction conditions were as follows:extracted 5 times with 50 times the amount of water for 15 minutes each time.Yields of total polysaccharides in P. igniarius from Dongbei mulberry

Dongbei birch

Gansu mulberry and Hunan mulberry were 5.37%

2.21%

3.04% and 3.35%

they all contained glucose and galactose;yield of total polysaccharides in P. igniarius from Shandong mulberry was 2.77%

which contained xylose

galactose

glucose and lactose;yield of total polysaccharides in P. igniarius from un-know place was 3.58%

which contained glucose. Conclusion: Microwave extraction method is stable with high extraction rate.The content of total polysaccharides in P. igniarius from Dongbei mulberry is highest by comparing with other origin and species

TLC can preliminary determine total polysaccharides from fruiting body of P. igniarius all contain glucose.