响应曲面法优化伸筋草总生物碱的微波辅助提取工艺

黄鹤; 刘有平; 王鑫; 邸欣

doi:10.13422/j.cnki.syfjx.2014180034

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响应曲面法优化伸筋草总生物碱的微波辅助提取工艺

工艺与制剂 | 更新时间：2024-01-04

- 响应曲面法优化伸筋草总生物碱的微波辅助提取工艺
- Optimization of Microwave-assisted Extraction Technology for Total Alkaloids from Lycopodii Herba by Response Surface Methodology
- 中国实验方剂学杂志 2014年20卷第18期页码：34-37
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- DOI：10.13422/j.cnki.syfjx.2014180034
  中图分类号： R284.2
- 纸质出版日期：2014
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黄鹤, 刘有平, 王鑫, 等. 响应曲面法优化伸筋草总生物碱的微波辅助提取工艺[J]. 中国实验方剂学杂志, 2014,20(18):34-37.

HUANG He, LIU You-ping, WANG Xin, et al. Optimization of Microwave-assisted Extraction Technology for Total Alkaloids from Lycopodii Herba by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 34-37.
黄鹤, 刘有平, 王鑫, 等. 响应曲面法优化伸筋草总生物碱的微波辅助提取工艺[J]. 中国实验方剂学杂志, 2014,20(18):34-37. DOI： 10.13422/j.cnki.syfjx.2014180034.

HUANG He, LIU You-ping, WANG Xin, et al. Optimization of Microwave-assisted Extraction Technology for Total Alkaloids from Lycopodii Herba by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 34-37. DOI： 10.13422/j.cnki.syfjx.2014180034.

摘要

目的：优化微波辅助提取伸筋草总生物碱的工艺条件。方法：采用酸性染料比色法测定总生物碱含量，以石松灵碱为指标成分。在单因素试验基础上，以总生物碱提取率为评价指标，选取液料比、提取温度和提取时间3个因素进行Box-Behnken中心组合设计，采用响应曲面法优化伸筋草总生物碱的微波辅助提取工艺。结果：伸筋草总生物碱的最佳提取工艺为加17倍量甲醇-水-盐酸（85：15：1）于72℃提取8 min；总生物碱提取率4.092 mg·g-1，与预测值4.070 mg·g-1的偏差较小。结论：与传统提取工艺相比，优选的微波辅助提取工艺具有时间短、提取率高、能量消耗低等优点。

Abstract

Objective: To optimize microwave-assisted extraction process conditions of total alkaloids from Lycopodii Herba. Method: Acid dye colorimetry was employed to determine the content of total alkaloids with lycodoline as index component.Based on single factor tests

effects of liquid-solid ratio

extraction temperature and time on yield of total alkaloids were investigated with a three-factor and three-level Box-Behnken central composite design

microwave-assisted extraction conditions of total alkaloids from Lycopodii Herba was optimized by response surface methodology and compared with traditional extraction technique. Result: Optimum conditions were as follows:extracted 8 min with 17 times the amount of methanol-water-hydrochloric acid (85:15:1) at 72℃;yield of total alkaloids was 4.092 mg·g-1 under these conditions

which was consistent with the theoretic value of 4.070 mg·g-1. Conclusion: Microwave-assisted extraction process has many advantages over conventional methods

such as time saving

high extraction ratio and low solvent consumption.

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