响应曲面法优化野生酸荔枝核中抗乙肝成分的提取工艺

陈华妮; 潘洁萍; 黄小丹; 李振中; 钱力

doi:10.13422/j.cnki.syfjx.2014180041

您当前的位置：

首页 >

文章列表页 >

响应曲面法优化野生酸荔枝核中抗乙肝成分的提取工艺

工艺与制剂 | 更新时间：2024-01-04

- 响应曲面法优化野生酸荔枝核中抗乙肝成分的提取工艺
- Optimization of Extraction Process for Anti Hepatitis B Consituents from Acid Litchi Semen by Response Surface Methodology
- 中国实验方剂学杂志 2014年20卷第18期页码：41-44
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西教育厅优秀人才计划项目（20124147）;广西高校大学生创新计划项目（QJCX201335）;广西教育厅科研项目（201010LX373，201106LX441，200103YB114）
- DOI：10.13422/j.cnki.syfjx.2014180041
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
陈华妮, 潘洁萍, 黄小丹, 等. 响应曲面法优化野生酸荔枝核中抗乙肝成分的提取工艺[J]. 中国实验方剂学杂志, 2014,20(18):41-44.

CHEN Hua-ni, PAN Jie-ping, HUANG Xiao-dan, et al. Optimization of Extraction Process for Anti Hepatitis B Consituents from Acid Litchi Semen by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 41-44.
陈华妮, 潘洁萍, 黄小丹, 等. 响应曲面法优化野生酸荔枝核中抗乙肝成分的提取工艺[J]. 中国实验方剂学杂志, 2014,20(18):41-44. DOI： 10.13422/j.cnki.syfjx.2014180041.

CHEN Hua-ni, PAN Jie-ping, HUANG Xiao-dan, et al. Optimization of Extraction Process for Anti Hepatitis B Consituents from Acid Litchi Semen by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 41-44. DOI： 10.13422/j.cnki.syfjx.2014180041.

摘要

目的：优选酸荔枝核中抗乙肝黄酮类化合物的提取工艺。方法：以芦丁为对照品，采用UV测定总黄酮含量。以总黄酮提取率为指标，温度、粉碎度、乙醇体积分数、料液比、提取时间、搅拌速度为自变量，在单因素试验基础上，通过Plackett-Burman试验筛选显著影响因子，利用Box-Behnken设计考察各自变量与响应值之间的关系，模拟得到二次多项式回归方程的预测模型，确定最佳提取工艺。结果：粉碎度和乙醇体积分数是影响荔枝核中总黄酮提取效率的显著因子，最佳提取工艺条件为粉碎度120目，加14倍量75%乙醇于60℃提取2 h，搅拌速度90 r·min-1；总黄酮平均提取率17.49%（RSD 0.63%）。结论：优选的提取工艺稳定可行，适用于酸荔枝核中抗乙肝总黄酮类化合物的工业化生产。

Abstract

Objective: To optimize extracting process of total flavonoids from acid Litchi Semen. Method: Taking rutin as standard

the content of total flavonoids was determined by UV.With yield of total flavonoids as index

on the basis of single factor tests

Box-Behnken and Plackett-Burman tests were adopted to optimize extraction process by taking temperature

grinding degree

ethanol concentration

liquid-solid ratio

extracting time and stirring speed as factors. Result: Grinding degree and ethanol concentration were significant factors

optimum extraction technology was as following:grinding degree of 120 mesh

extracted 2 h with 14 times the amount of 75% ethanol at 60℃

stirring speed of 90 r·min-1.Under these conditions

average extraction rate of total flavonoids was 17.49% with RSD of 0.63%. Conclusion: This optimized technology is stable and feasible

which is suitable for industrial production of anti-hepatitis B flavonoids from acid Litchi Semen.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

响应面法优化牛舌草总黄酮的超声提取工艺

参葛补肾胶囊及其多糖黄酮对幼龄小鼠性早熟的影响

牡丹花总黄酮对高尿酸血症大鼠降尿酸作用

5种大孔吸附树脂对淫羊藿总黄酮的吸附特性考察